| Literature DB >> 27242142 |
Lihua Chen1, Qianqian Bu1, Huan Xu1, Yuan Liu1, Pengfei She1, Ruichen Tan1, Yong Wu2.
Abstract
Enterococcus faecalis (E. faecalis) is one of the major causes of biofilm infections. Berberine hydrochloride (BBH) has diverse pharmacological effects; however, the effects and mechanisms of BBH on E. faecalis biofilm formation and dispersion have not been reported. In this study, 99 clinical isolates from the urine samples of patients with urinary tract infections (UTIs) were collected and identified. Ten strains of E. faecalis with biofilm formation ability were studied. BBH inhibited E. faecalis biofilm formation and promoted the biofilm dispersion of E. faecalis. In addition, sortase A and esp expression levels were elevated during early E. faecalis biofilm development, whereas BBH significantly reduced their expression levels. The results of this study indicated that BBH effectively prevents biofilm formation and promotes biofilm dispersion in E. faecalis, most likely by inhibiting the expressions of sortase A and esp.Entities:
Keywords: Berberine hydrochloride; Biofilm; Confocal laser scanning microscopy; Enterococcus faecalis; esp; sortase A
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Year: 2016 PMID： 27242142 DOI： 10.1016/j.micres.2016.03.003
Source DB: PubMed Journal: Microbiol Res ISSN： 0944-5013 Impact factor: 5.415