Literature DB >> 27242007

Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins.

A Abdulmawjood1, J Wickhorst2, O Hashim3, O Sammra2, A A Hassan2, M Alssahen2, C Lämmler2, E Prenger-Berninghoff4, G Klein3.   

Abstract

In the present study 28 Trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene cpn60 encoding chaperonin. No cross reaction could be observed with control strains representing four species of genus Trueperella and seven species of closely related genus Arcanobacterium. The present cpn60 LAMP assay might allow a reliable and low cost identification of T. pyogenes also in laboratories with less specified equipment.
Copyright © 2016 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  LAMP; Loop-mediated isothermal amplification; Real-time fluorometer; Trueperella pyogenes

Mesh：

Year:  2016        PMID： 27242007     DOI： 10.1016/j.mcp.2016.05.003

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  5 in total

1.  Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus).

Authors:  Jörn-Peter Wickhorst; Abdulwahed Ahmed Hassan; Omar Hashim Sheet; Tobias Eisenberg; Osama Sammra; Mazen Alssahen; Christoph Lämmler; Ellen Prenger-Berninghoff; Michael Zschöck; Markus Timke; Amir Abdulmawjood
Journal:  Folia Microbiol (Praha)       Date:  2017-05-23       Impact factor: 2.099

2.  First isolation of Arcanobacterium pinnipediorum from a grey seal pup (Halichoerus grypus) in the UK.

Authors:  Mazen Alssahen; Geoffrey Foster; Abdulwahed Ahmed Hassan; Jörg Rau; Christoph Lämmler; Ellen Prenger-Berninghoff; Tobias Eisenberg; Mathew Robinson; Amir Abdulmawjood
Journal:  Folia Microbiol (Praha)       Date:  2021-11-26       Impact factor: 2.099

3.  Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay.

Authors:  Marwa F E Ahmed; Mazen Alssahen; Christoph Lämmler; Bernd Köhler; Martin Metzner; Madeleine Plötz; Amir Abdulmawjood
Journal:  Folia Microbiol (Praha)       Date:  2021-11-15       Impact factor: 2.099

4.  Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion.

Authors:  Gamal Wareth; Mohamed El-Diasty; Falk Melzer; Jayaseelan Murugaiyan; Amir Abdulmawjood; Lisa D Sprague; Heinrich Neubauer
Journal:  Vet Med Int       Date:  2018-03-20

5.  Studies on Trueperella pyogenes isolated from an okapi (Okapia johnstoni) and a royal python (Python regius).

Authors:  Marwa F E Ahmed; Mazen Alssahen; Christoph Lämmler; Tobias Eisenberg; Madeleine Plötz; Amir Abdulmawjood
Journal:  BMC Vet Res       Date:  2020-08-14       Impact factor: 2.741
  5 in total

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