| Literature DB >> 27236806 |
An Coosemans1,2, Sandra Tuyaerts3, Kim Morias4, Jurgen Corthals5, Carlo Heirman5, Kris Thielemans5, Stefaan W Van Gool6, Ignace Vergote3,7, Frédéric Amant3,7.
Abstract
The immune system is a crucial player in the development of cancer. Once it is in imbalance and immunosuppressive mechanisms supporting tumor growth take over control, dendritic cell immunotherapy might offer a solution to restore the balance. There are several methods to manufacture dendritic cells but none of them has yet proven to be superior to others. In this chapter, we discuss the methodology using electroporation of mRNA encoding Wilms' tumor gene 1, survivin, and TriMix (mixture of caTLR4, CD40L, and CD70) to simultaneously load and mature dendritic cells.Entities:
Keywords: Dendritic cell; Electroporation; Survivin; TriMix; WT1; mRNA
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Year: 2016 PMID： 27236806 DOI： 10.1007/978-1-4939-3625-0_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745