| Literature DB >> 27236685 |
Christina Barckhausen1, Brent Rice2, Stefano Baila3, Luc Sensebé4, Hubert Schrezenmeier5,6, Philipp Nold1, Holger Hackstein7, Markus Thomas Rojewski8,9.
Abstract
This chapter describes a method for GMP-compliant expansion of human mesenchymal stromal/stem cells (hMSC) from bone marrow aspirates, using the Quantum(®) Cell Expansion System from Terumo BCT. The Quantum system is a functionally closed, automated hollow fiber bioreactor system designed to reproducibly grow cells in either GMP or research laboratory environments. The chapter includes protocols for preparation of media, setup of the Quantum system, coating of the hollow fiber bioreactor, as well as loading, feeding, and harvesting of cells. We suggest a panel of quality controls for the starting material, the interim product, as well as the final product.Entities:
Keywords: Bioreactor; Clinical application; Functionally closed; GMP; Glucose consumption; Hollow fiber; Lactate generation; MSC; Mesenchymal stem cells; Mesenchymal stromal cells; Platelet lysate
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Year: 2016 PMID： 27236685 DOI： 10.1007/978-1-4939-3584-0_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745