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Literature DB >> 27233749

A chemiluminescent platform for smartphone monitoring of H₂O₂ in human exhaled breath condensates.

Miguel E Quimbar¹, Katherine M Krenek¹, Alexander R Lippert².

Abstract

Noninvasive measurement of oxidative markers in clinical samples has the potential to rapidly provide information for disease management, but is limited by the need for expensive analytical instrumentation that precludes home monitoring or point-of-care applications. We have developed a simple to use diagnostic platform for airway hydrogen peroxide (H2O2) that combines optimized reaction-based chemiluminescent designs with an inexpensive home-built darkbox and readily available smartphone cameras. Specialized photography software applications and analysis of pixel intensity enables quantification of sample concentrations. Using this platform, sample H2O2 concentrations as low as 264nM can be detected. The platform has been used to measure H2O2 in the exhaled breath condensates of human subjects, showing good agreement with the standard Amplex Red assay.

Entities: Chemical Disease Gene Species

Keywords: Chemiluminescence; Exhaled breath condensates; Hydrogen peroxide; Point-of-care; Smartphone

Mesh：

Substances：

Year: 2016 PMID： 27233749 PMCID： PMC5075522 DOI： 10.1016/j.ymeth.2016.05.017

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

50 in total

1. Design and synthesis of chemiluminescent probes for the detection of cholinesterase activity.

Authors: Stéphane Sabelle; Pierre-Yves Renard; Karine Pecorella; Sophie de Suzzoni-Dézard; Christophe Créminon; Jacques Grassi; Charles Mioskowski
Journal: J Am Chem Soc Date: 2002-05-01 Impact factor: 15.419

2. Boronate oxidation as a bioorthogonal reaction approach for studying the chemistry of hydrogen peroxide in living systems.

Authors: Alexander R Lippert; Genevieve C Van de Bittner; Christopher J Chang
Journal: Acc Chem Res Date: 2011-08-11 Impact factor: 22.384

3. [Horseradish peroxidase: kinetic studies and optimization of peroxidase activity determination using the substrates H2O2 and 3,3',5,5'-tetramethylbenzidine].

Authors: H Gallati; I Pracht
Journal: J Clin Chem Clin Biochem Date: 1985-08

4. Expired breath hydrogen peroxide is a marker of acute airway inflammation in pediatric patients with asthma.

Authors: A W Dohlman; H R Black; J A Royall
Journal: Am Rev Respir Dis Date: 1993-10

5. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.

Authors: M Zhou; Z Diwu; N Panchuk-Voloshina; R P Haugland
Journal: Anal Biochem Date: 1997-11-15 Impact factor: 3.365

A chemiluminescent platform for smartphone monitoring of H₂O₂ in human exhaled breath condensates.

1. Design and synthesis of chemiluminescent probes for the detection of cholinesterase activity.

2. Boronate oxidation as a bioorthogonal reaction approach for studying the chemistry of hydrogen peroxide in living systems.

3. [Horseradish peroxidase: kinetic studies and optimization of peroxidase activity determination using the substrates H2O2 and 3,3',5,5'-tetramethylbenzidine].

4. Expired breath hydrogen peroxide is a marker of acute airway inflammation in pediatric patients with asthma.

5. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.

6. In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles.

7. Combined use of exhaled hydrogen peroxide and nitric oxide in monitoring asthma.

8. Mobile phones democratize and cultivate next-generation imaging, diagnostics and measurement tools.

Review 9. CMOS cell sensors for point-of-care diagnostics.

10. Detection of hydrogen peroxide with chemiluminescent micelles.

1. A Chemiluminescent Probe for HNO Quantification and Real-Time Monitoring in Living Cells.

2. Chemiluminescent Measurement of Hydrogen Peroxide in the Exhaled Breath Condensate of Healthy and Asthmatic Adults.

Review 3. Activity-Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond.

4. Energy transfer chemiluminescence for ratiometric pH imaging.

5. A chemiluminescent probe for cellular peroxynitrite using a self-immolative oxidative decarbonylation reaction.

6. Seeking Illumination: The Path to Chemiluminescent 1,2-Dioxetanes for Quantitative Measurements and In Vivo Imaging.