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Literature DB >> 27233264

Infectious Viral Quantification of Chikungunya Virus-Virus Plaque Assay.

Parveen Kaur¹, Regina Ching Hua Lee¹, Justin Jang Hann Chu².

Abstract

The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays.

Entities: Disease Species

Keywords: Aquacide; Carboxymethyl cellulose; Chikungunya virus; Crystal violet; Plaque assays; Virus titration

Mesh：

Year: 2016 PMID： 27233264 DOI： 10.1007/978-1-4939-3618-2_9

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

7 in total

1. Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes.

Authors: Lyudmyla G Glushakova; Barry W Alto; Myong-Sang Kim; Keenan Wiggins; Bradley Eastmond; Patricia Moussatche; Nathan D Burkett-Cadena; Steven A Benner
Journal: J Virol Methods Date: 2018-08-09 Impact factor: 2.014

2. Identification of small molecule inhibitors of the Chikungunya virus nsP1 RNA capping enzyme.

Authors: Kristen M Feibelman; Benjamin P Fuller; Linfeng Li; Daniel V LaBarbera; Brian J Geiss
Journal: Antiviral Res Date: 2018-04-20 Impact factor: 5.970

3. Laboratory Evaluation of Commercially Available Platforms to Detect West Nile and Zika Viruses From Honey Cards.

Authors: Kristen L Burkhalter; Keenan Wiggins; Nathan Burkett-Cadena; Barry W Alto
Journal: J Med Entomol Date: 2018-05-04 Impact factor: 2.278

4. Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

Authors: Ana Chumbe; Ray Izquierdo-Lara; Katherine Calderón; Manolo Fernández-Díaz; Vikram N Vakharia
Journal: Virol J Date: 2017-11-23 Impact factor: 4.099

Infectious Viral Quantification of Chikungunya Virus-Virus Plaque Assay.

1. Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes.

2. Identification of small molecule inhibitors of the Chikungunya virus nsP1 RNA capping enzyme.

3. Laboratory Evaluation of Commercially Available Platforms to Detect West Nile and Zika Viruses From Honey Cards.

4. Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein.

5. Chikungunya virus vector competency of Brazilian and Florida mosquito vectors.

6. Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine.

7. Experimental Vertical Transmission of Chikungunya Virus by Brazilian and Florida Aedes Albopictus Populations.