Akiko Onda1, Hajime Kono2, Qibin Jiao3, Takayuki Akimoto4, Toshikazu Miyamoto5, Yasuhiro Sawada6, Katsuhiko Suzuki7, Yoichiro Kusakari8, Susumu Minamisawa8, Toru Fukubayashi7. 1. Department of Internal Medicine, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan. 2. Department of Internal Medicine, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan. kono@med.teikyo-u.ac.jp. 3. Department of Cardiology, Hangzhou Cardiovascular Disease Institute, Hangzhou Normal University, Hangzhou, Zhejiang Province, China. 4. Center for Disease Biology and Integrative Medicine, The University of Tokyo, Japan. 5. Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan. 6. Department of Rehabilitation for the Movement Functions, Research Institute, National Rehabilitation Center for Persons with Disabilities, Saitama, Japan. 7. Faculty of Sport Sciences, Waseda University, Saitama, Japan. 8. Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan.
Abstract
INTRODUCTION: Disuse-induced skeletal muscle atrophy is a serious concern; however, there is not an effective mouse model to elucidate the molecular mechanisms. We developed a noninvasive atrophy model in mice. METHODS: After the ankle joints of mice were bandaged into a bilateral plantar flexed position, either bilateral or unilateral hindlimbs were immobilized by wrapping in bonsai steel wire. RESULTS: After 3, 5, or 10 days of immobilization of the hip, knee, and ankle, the weight of the soleus and plantaris muscles decreased significantly in both bilateral and unilateral immobilization. MAFbx/atrogin-1 and MuRF1 mRNA was found to have significantly increased in both muscles, consistent with disuse-induced atrophy. Notably, the procedure did not result in either edema or necrosis in the fixed hindlimbs. CONCLUSIONS: This method allows repeated, direct access to the immobilized muscle, making it a useful procedure for concurrent application and assessment of various therapeutic interventions. Muscle Nerve 54: 788-791, 2016.
INTRODUCTION: Disuse-induced skeletal muscle atrophy is a serious concern; however, there is not an effective mouse model to elucidate the molecular mechanisms. We developed a noninvasive atrophy model in mice. METHODS: After the ankle joints of mice were bandaged into a bilateral plantar flexed position, either bilateral or unilateral hindlimbs were immobilized by wrapping in bonsai steel wire. RESULTS: After 3, 5, or 10 days of immobilization of the hip, knee, and ankle, the weight of the soleus and plantaris muscles decreased significantly in both bilateral and unilateral immobilization. MAFbx/atrogin-1 and MuRF1 mRNA was found to have significantly increased in both muscles, consistent with disuse-induced atrophy. Notably, the procedure did not result in either edema or necrosis in the fixed hindlimbs. CONCLUSIONS: This method allows repeated, direct access to the immobilized muscle, making it a useful procedure for concurrent application and assessment of various therapeutic interventions. Muscle Nerve 54: 788-791, 2016.
Authors: William P Mayer; Josemberg da S Baptista; Flavia De Oliveira; Matsuyoshi Mori; Edson A Liberti Journal: Histochem Cell Biol Date: 2021-09-02 Impact factor: 4.304