| Literature DB >> 27226077 |
Eun-Mi Lee1,2, Ah-Young Kim1,2, Eun-Joo Lee1,2, Jin-Kyu Park1, Se-Il Park3, Ssang-Goo Cho4, Hong Kyun Kim5, Shin-Yoon Kim6, Kyu-Shik Jeong1,2.
Abstract
Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/ mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.Entities:
Keywords: Horse; Induced pluripotent stem cells (iPSCs); Muscle regeneration; Transplantation
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Year: 2016 PMID： 27226077 DOI： 10.3727/096368916X691691
Source DB: PubMed Journal: Cell Transplant ISSN： 0963-6897 Impact factor: 4.064