BACKGROUND: Non-ablative fractional lasers have been effectively used in skin rejuvenation. OBJECTIVES: Evaluate the efficacy of 1550 nm Erbium Glass Laser for facial rejuvenation through the correlation of clinical evaluation and histopathology, immunohistochemistry and electron microscopy analysis. METHODS: Fifteen subjects (average age 56.4 years old, skin types II-III) with mild to moderate photodamage were submitted to biopsies and 3 facial treatments. Data from the photo assessments and the wrinkles improvement were analyzed 4 months after the treatments. The biopsy skins were fixed in neutral buffered formalin before paraffin embedding, and hematoxylin and eosin stained. The histomorphometric quantification of collagen and elastic fibers; intercellular adhesion molecule 1 expression by immunohistochemistry; and analysis of cell cycle phases, the electrical potential of the mitochondrial and IL- 1, CD34, TGF-β and caspase-3 expression by flow cytometry were analyzed. RESULTS: After 4 months of treatment, collagen fibers had increased by 6.68%, and ICAM-1 by 4.47% in vessel area. Significantly enhanced IL-1 and TGF-β receptor expression were identified after treatment. Proliferative responses and non-apoptosis-dependent caspase-3 activity were both observed in the cell after dermal treatment. CONCLUSION: The histopathology, immunohistochemistry and electron microscopy showed an improvement compatible to the clinical effectiveness after 4 months.
BACKGROUND: Non-ablative fractional lasers have been effectively used in skin rejuvenation. OBJECTIVES: Evaluate the efficacy of 1550 nm Erbium Glass Laser for facial rejuvenation through the correlation of clinical evaluation and histopathology, immunohistochemistry and electron microscopy analysis. METHODS: Fifteen subjects (average age 56.4 years old, skin types II-III) with mild to moderate photodamage were submitted to biopsies and 3 facial treatments. Data from the photo assessments and the wrinkles improvement were analyzed 4 months after the treatments. The biopsy skins were fixed in neutral buffered formalin before paraffin embedding, and hematoxylin and eosin stained. The histomorphometric quantification of collagen and elastic fibers; intercellular adhesion molecule 1 expression by immunohistochemistry; and analysis of cell cycle phases, the electrical potential of the mitochondrial and IL- 1, CD34, TGF-β and caspase-3 expression by flow cytometry were analyzed. RESULTS: After 4 months of treatment, collagen fibers had increased by 6.68%, and ICAM-1 by 4.47% in vessel area. Significantly enhanced IL-1 and TGF-β receptor expression were identified after treatment. Proliferative responses and non-apoptosis-dependent caspase-3 activity were both observed in the cell after dermal treatment. CONCLUSION: The histopathology, immunohistochemistry and electron microscopy showed an improvement compatible to the clinical effectiveness after 4 months.
Authors: Márcia Farina Kamilos; Lana Maria Aguiar; Valéria Holmo Batista; Cristiane Lima Roa; Fernando Nalesso Aguiar; José Maria Soares Júnior; Edmund Chada Baracat Journal: Clinics (Sao Paulo) Date: 2021-03-26 Impact factor: 2.365