Marlos Barbosa-Ribeiro1, Adriana De-Jesus-Soares1, Alexandre A Zaia1, Caio C R Ferraz1, José F A Almeida1, Brenda P F A Gomes2. 1. Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil. 2. Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil. Electronic address: bpgomes@unicamp.br.
Abstract
INTRODUCTION: The aim of this study was to investigate the prevalence of virulence factors and the antimicrobial resistance of Enterococcus faecalis isolates of teeth with failure of the endodontic treatment. METHODS: Twenty root canal samples were collected from teeth with apical periodontitis. E. faecalis was firstly identified based on phenotypic features and then by 16S ribosomal RNA gene sequencing. The antimicrobial susceptibility was determined by the minimum inhibitory concentration (MIC) of amoxicillin, amoxicillin + clavulanate, azithromycin, benzylpenicillin, ciprofloxacin, clindamycin, chloramphenicol, doxycycline, erythromycin, gentamicin, metronidazole, moxifloxacin, rifampicin, tetracycline, and vancomycin using the E test method. Virulence factors (ace, asa, asa373, cylA, efaA, esp, and gelE) were detected by polymerase chain reaction assay. RESULTS: Amoxicillin + clavulanate was effective against all strains. Intermediate and total resistance was found against the majority of the tested antimicrobials. The susceptibility of some microorganisms to some antimicrobial agents changed according to the evaluation time. MIC50 and MIC90 also varied according to the evaluation time. In relation to the virulence factors of the E faecalis isolates, ace was detected in 100% of the strains, asa (60%), asa373 (15%), efaA (95%), esp (70%), and gelE (75%), whereas cylA was not detected. CONCLUSIONS: It was concluded that E. faecalis isolates from persistent endodontic infections showed varied degrees of intermediate/total resistance to several antimicrobial agents, with amoxicillin + clavulanate being the most effective agent. Moreover, the strains showed different patterns for virulence gene detection.
INTRODUCTION: The aim of this study was to investigate the prevalence of virulence factors and the antimicrobial resistance of Enterococcus faecalis isolates of teeth with failure of the endodontic treatment. METHODS: Twenty root canal samples were collected from teeth with apical periodontitis. E. faecalis was firstly identified based on phenotypic features and then by 16S ribosomal RNA gene sequencing. The antimicrobial susceptibility was determined by the minimum inhibitory concentration (MIC) of amoxicillin, amoxicillin + clavulanate, azithromycin, benzylpenicillin, ciprofloxacin, clindamycin, chloramphenicol, doxycycline, erythromycin, gentamicin, metronidazole, moxifloxacin, rifampicin, tetracycline, and vancomycin using the E test method. Virulence factors (ace, asa, asa373, cylA, efaA, esp, and gelE) were detected by polymerase chain reaction assay. RESULTS:Amoxicillin + clavulanate was effective against all strains. Intermediate and total resistance was found against the majority of the tested antimicrobials. The susceptibility of some microorganisms to some antimicrobial agents changed according to the evaluation time. MIC50 and MIC90 also varied according to the evaluation time. In relation to the virulence factors of the E faecalis isolates, ace was detected in 100% of the strains, asa (60%), asa373 (15%), efaA (95%), esp (70%), and gelE (75%), whereas cylA was not detected. CONCLUSIONS: It was concluded that E. faecalis isolates from persistent endodontic infections showed varied degrees of intermediate/total resistance to several antimicrobial agents, with amoxicillin + clavulanate being the most effective agent. Moreover, the strains showed different patterns for virulence gene detection.
Authors: Priscila Amanda Francisco; Pedro Ivo da Graça Fagundes; João Carlos Lemes-Junior; Augusto Rodrigues Lima; Maicon Ricardo Zieberg Passini; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2021-02-09 Impact factor: 3.573
Authors: Marlos Barbosa-Ribeiro; Rodrigo Arruda-Vasconcelos; Lidiane M Louzada; Danielle G Dos Santos; Fernando D Andreote; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2020-08-28 Impact factor: 3.573
Authors: Marlos Barbosa-Ribeiro; Rodrigo Arruda-Vasconcelos; Adriana de-Jesus-Soares; Alexandre Augusto Zaia; Caio Cezar Randi Ferraz; José Flávio Affonso de Almeida; Brenda P F A Gomes Journal: Clin Oral Investig Date: 2018-10-26 Impact factor: 3.573
Authors: Manuela Kaufmann; Patrik Lenherr; Clemens Walter; Thomas Thurnheer; Thomas Attin; Daniel B Wiedemeier; Patrick R Schmidlin Journal: Dent J (Basel) Date: 2018-10-20