Literature DB >> 2721917

Purification of a TDH-related hemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus 06: K46.

T Honda1, Y Ni, T Miwatani.   

Abstract

A hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus 06: K46 was purified by 55% ammonium sulfate fractionation and successive column chromatographies on DEAE-cellulose, hydroxyapatite, Sepharose 4B and Mono Q. The purified hemolysin was physicochemically and immunologically identical with the Vp-TRH (V. parahaemolyticus thermostable direct hemolysin related hemolysin) recently described in V. parahaemolyticus 03: K6 (Honda et al. Infect. Immun. 56: 961-965, 1988). This indicates that V. parahaemolyticus of Kanagawa-negative clinical isolates possessing not only 03: K6 but also different serotypes such as 06: K46 produce Vp-TRH. Production of Vp-TRH by most clinical isolates of Kanagawa-negative V. parahaemolyticus was also demonstrated. These results suggest the importance of Vp-TRH among clinical isolates of Kanagawa-negative V. parahaemolyticus.

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Year:  1989        PMID： 2721917     DOI： 10.1111/j.1574-6968.1989.tb03307.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  9 in total

1.  Incidence of Vibrio parahaemolyticus in U.S. coastal waters and oysters.

Authors:  A DePaola; L H Hopkins; J T Peeler; B Wentz; R M McPhearson
Journal:  Appl Environ Microbiol       Date:  1990-08       Impact factor: 4.792

2.  Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis.

Authors:  H Shirai; H Ito; T Hirayama; Y Nakamoto; N Nakabayashi; K Kumagai; Y Takeda; M Nishibuchi
Journal:  Infect Immun       Date:  1990-11       Impact factor: 3.441

3.  Serologic and molecular characterization of Vibrio parahaemolyticus strains isolated from seawater and fish products of the Gulf of Mexico.

Authors:  María Eugenia Cabrera-García; Carlos Vázquez-Salinas; Elsa Irma Quiñones-Ramírez
Journal:  Appl Environ Microbiol       Date:  2004-11       Impact factor: 4.792

4.  Effects of iron limitation on production of a siderophore, outer membrane proteins, and hemolysin and on hydrophobicity, cell adherence, and lethality for mice of Vibrio parahaemolyticus.

Authors:  J H Dai; Y S Lee; H C Wong
Journal:  Infect Immun       Date:  1992-07       Impact factor: 3.441

5.  Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)

Authors:  M Xu; K Yamamoto; T Honda; M ] Ming X [corrected to Xu
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490

6.  Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes.

Authors:  Linda N Ward; Asim K Bej
Journal:  Appl Environ Microbiol       Date:  2006-03       Impact factor: 4.792

7.  Production of monoclonal antibodies against thermostable direct hemolysin of Vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay.

Authors:  T Honda; Y Ni; M Yoh; T Miwatani
Journal:  Med Microbiol Immunol       Date:  1989       Impact factor: 3.402

8.  Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by a clinically isolated Vibrio parahaemolyticus strain, TH3766.

Authors:  M Xu; T Iida; K Yamamoto; Y Takarada; T Miwatani; T Honda
Journal:  Infect Immun       Date:  1994-01       Impact factor: 3.441

9.  A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh+ , tdh+ , and ureR + ).

Authors:  Ben Niu; Bin Hong; Zhaohuan Zhang; Lili Mu; Pradeep K Malakar; Haiquan Liu; Yingjie Pan; Yong Zhao
Journal:  Front Microbiol       Date:  2018-08-02       Impact factor: 5.640
  9 in total

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