| Literature DB >> 27217303 |
Dimitrios Kouroupis1, Athena Kyrkou1, Eleni Triantafyllidi2, Michalis Katsimpoulas3, George Chalepakis4, Anna Goussia2, Anastasios Georgoulis5, Carol Murphy6, Theodore Fotsis7.
Abstract
In the present study, we combined stem cell technology with a non-absorbable biomaterial for the reconstruction of the ruptured ACL. Towards this purpose, multipotential stromal cells derived either from subcutaneous human adipose tissue (hAT-MSCs) or from induced pluripotent stem cells (iPSCs) generated from human foreskin fibroblasts (hiPSC-MSCs) were cultured on the biomaterial for 21days in vitro to generate a 3D bioartifical ACL graft. Stem cell differentiation towards bone and ligament at the ends and central part of the biomaterial was selectively induced using either BMP-2/FGF-2 or TGF-β/FGF-2 combinations, respectively. The bioartificial ACL graft was subsequently implanted in a swine ACL rupture model in place of the surgically removed normal ACL. Four months post-implantation, the tissue engineered ACL graft generated an ACL-like tissue exhibiting morphological and biochemical characteristics resembling those of normal ACL.Entities:
Keywords: Anterior cruciate ligament reconstruction; Bioartificial ACL graft; Biomaterials; Human adipose tissue multipotential stromal cells; Human induced pluripotent stem cells; Tissue engineering
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Year: 2016 PMID： 27217303 DOI： 10.1016/j.scr.2016.04.016
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020