Literature DB >> 27216880

Targeted nanobubbles in low-frequency ultrasound-mediated gene transfection and growth inhibition of hepatocellular carcinoma cells.

Bolin Wu1, Qiang Qiao1, Xue Han1, Hui Jing1, Hao Zhang2, Hongjian Liang2, Wen Cheng3.   

Abstract

The use of SonoVue combined with ultrasound exposure increases the transfection efficiency of short interfering RNA (siRNA). The objective of this study was to prepare targeted nanobubbles (TNB) conjugated with NET-1 siRNA and an antibody GPC3 to direct nanobubbles to hepatocellular carcinoma cells. SMMC-7721 human hepatocellular carcinoma cells were treated with six different groups. The transfection efficiency and cellular apoptosis were measured by flow cytometry. The protein and messenger RNA (mRNA) expression were measured by Western blot and quantitative real-time PCR, respectively. The migration and invasion potential of the cells were determined by Transwell analysis. The results show that US-guided siRNA-TNB transfection effectively enhanced gene silencing. In summary, siRNA-TNB may be an effective delivery vector to mediate highly effective RNA interference in tumor treatment.

Entities:  

Keywords:  Hepatocellular carcinoma; Nanobubbles; Ultrasound; siRNA

Mesh：

Substances：

Year:  2016        PMID： 27216880     DOI： 10.1007/s13277-016-5082-2

Source DB:  PubMed          Journal:  Tumour Biol        ISSN： 1010-4283


  35 in total

1.  Neuroepithelial transforming protein 1 short interfering RNA-mediated gene silencing with microbubble and ultrasound exposure inhibits the proliferation of hepatic carcinoma cells in vitro.

Authors:  Xue Han; Wen Cheng; Hui Jing; Jiu-Wei Zhang; Li-Li Tang
Journal:  J Ultrasound Med       Date:  2012-06       Impact factor: 2.153

2.  Enhanced delivery of monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly l-lysine nanoparticles loading platelet-derived growth factor BB small interfering RNA by ultrasound and/or microbubbles to rat retinal pigment epithelium cells.

Authors:  Jing Du; Qiu Sheng Shi; Ying Sun; Pei Feng Liu; Ming Jie Zhu; Lian Fang Du; You Rong Duan
Journal:  J Gene Med       Date:  2011-06       Impact factor: 4.565

3.  Diagnostic value of HSP70, glypican 3, and glutamine synthetase in hepatocellular nodules in cirrhosis.

Authors:  Luca Di Tommaso; Giada Franchi; Young Nyun Park; Barbara Fiamengo; Annarita Destro; Emanuela Morenghi; Marco Montorsi; Guido Torzilli; Maurizio Tommasini; Luigi Terracciano; Luigi Tornillo; Raffaella Vecchione; Massimo Roncalli
Journal:  Hepatology       Date:  2007-03       Impact factor: 17.425

4.  Formulation and characterization of echogenic lipid-Pluronic nanobubbles.

Authors:  Tianyi M Krupka; Luis Solorio; Robin E Wilson; Hanping Wu; Nami Azar; Agata A Exner
Journal:  Mol Pharm       Date:  2010-02-01       Impact factor: 4.939

5.  Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells.

Authors:  Shufang Chang; Juan Guo; Jiangchuan Sun; Shenyin Zhu; Yu Yan; Yi Zhu; Min Li; Zhigang Wang; Ronald X Xu
Journal:  Ultrason Sonochem       Date:  2012-07-05       Impact factor: 7.491

6.  Enhancement of survivin gene downregulation and cell apoptosis by a novel combination: liposome microbubbles and ultrasound exposure.

Authors:  Zhiyi Chen; Kun Liang; Jianhua Liu; Mingxing Xie; Xinfang Wang; Qing Lü; Jing Zhang; Lingyun Fang
Journal:  Med Oncol       Date:  2009-01-07       Impact factor: 3.064

7.  Microbubbles targeted to intercellular adhesion molecule-1 bind to activated coronary artery endothelial cells.

Authors:  F S Villanueva; R J Jankowski; S Klibanov; M L Pina; S M Alber; S C Watkins; G H Brandenburger; W R Wagner
Journal:  Circulation       Date:  1998-07-07       Impact factor: 29.690

8.  Lipid-shelled vehicles: engineering for ultrasound molecular imaging and drug delivery.

Authors:  Katherine W Ferrara; Mark A Borden; Hua Zhang
Journal:  Acc Chem Res       Date:  2009-07-21       Impact factor: 22.384

9.  Repurposing the CRISPR-Cas9 system for targeted DNA methylation.

Authors:  Aleksandar Vojta; Paula Dobrinić; Vanja Tadić; Luka Bočkor; Petra Korać; Boris Julg; Marija Klasić; Vlatka Zoldoš
Journal:  Nucleic Acids Res       Date:  2016-03-11       Impact factor: 16.971

Review 10.  Gene therapy for cardiovascular disease mediated by ultrasound and microbubbles.

Authors:  Zhi-Yi Chen; Yan Lin; Feng Yang; Lan Jiang; Shu ping Ge
Journal:  Cardiovasc Ultrasound       Date:  2013-04-17       Impact factor: 2.062
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  12 in total

1.  High-Frequency Array-Based Nanobubble Nonlinear Imaging in a Phantom and In Vivo.

Authors:  Carly Pellow; Emmanuel Cherin; Eric C Abenojar; Agata A Exner; Gang Zheng; Christine E M Demore; David E Goertz
Journal:  IEEE Trans Ultrason Ferroelectr Freq Control       Date:  2021-05-25       Impact factor: 3.267

Review 2.  Trigger-Responsive Gene Transporters for Anticancer Therapy.

Authors:  Santhosh Kalash Rajendrakumar; Saji Uthaman; Chong Su Cho; In-Kyu Park
Journal:  Nanomaterials (Basel)       Date:  2017-05-26       Impact factor: 5.076

3.  Ultrasound microbubbles mediated miR-let-7b delivery into CD133+ ovarian cancer stem cells.

Authors:  Chaopin Yang; Bingcheng Li; Jinsui Yu; Feng Yang; Kuan Cai; Zhiyi Chen
Journal:  Biosci Rep       Date:  2018-09-28       Impact factor: 3.840

4.  VTIQ evaluates antitumor effects of NET-1 siRNA by UTMD in HCC xenograft models.

Authors:  Xitian Liang; Bolin Wu; Haitao Shang; Xue Han; Hui Jing; Yixin Sun; Wen Cheng
Journal:  Oncol Lett       Date:  2018-06-19       Impact factor: 2.967

5.  The better effects of microbubble ultrasound transfection of miR-940 on cell proliferation inhibition and apoptosis promotion in human cervical cancer cells.

Authors:  Xiaojun Xiao; Yujuan Zhang; Qi Lin; Keli Zhong
Journal:  Onco Targets Ther       Date:  2019-08-21       Impact factor: 4.147

6.  Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner.

Authors:  Hiroshi Kida; Koyo Nishimura; Koki Ogawa; Akiko Watanabe; Loreto B Feril; Yutaka Irie; Hitomi Endo; Shigeru Kawakami; Katsuro Tachibana
Journal:  Front Pharmacol       Date:  2020-04-01       Impact factor: 5.810

7.  Quantitative Proteomics Analysis of FFPE Tumor Samples Reveals the Influences of NET-1 siRNA Nanoparticles and Sonodynamic Therapy on Tetraspanin Protein Involved in HCC.

Authors:  Bolin Wu; Haitao Shang; Jiayin Liu; Xitian Liang; Yanchi Yuan; Yichi Chen; Chunyue Wang; Hui Jing; Wen Cheng
Journal:  Front Mol Biosci       Date:  2021-05-10

8.  Cavitation-threshold Determination and Rheological-parameters Estimation of Albumin-stabilized Nanobubbles.

Authors:  Maxime Lafond; Akiko Watanabe; Shin Yoshizawa; Shin-Ichiro Umemura; Katsuro Tachibana
Journal:  Sci Rep       Date:  2018-05-10       Impact factor: 4.379

9.  Evaluation of therapeutic effect of targeting nanobubbles conjugated with NET-1 siRNA by shear wave elastography: an in vivo study of hepatocellular carcinoma bearing mice model.

Authors:  Haitao Shang; Bolin Wu; Xitian Liang; Yixin Sun; Xue Han; Lei Zhang; Qiucheng Wang; Wen Cheng
Journal:  Drug Deliv       Date:  2019-12       Impact factor: 6.419

10.  Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis.

Authors:  Hui Luo; Jian Li; Qi Lin; Xiaojun Xiao; Yang Shi; Xiuqin Ye; Zhanghong Wei; Yingying Liu; Jinfeng Xu
Journal:  Biosci Rep       Date:  2020-11-27       Impact factor: 3.840
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