Ahmed H F El-Tawdi1, Marwa Matboli2, Sarah El-Nakeep3, Ahmed E M Azazy4, Omar Abdel-Rahman5. 1. a General and Plastic Surgery Department , Military Medical Academy , Cairo , Egypt. 2. b Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt. 3. c Hepatology and Gastroenterology Unit, Internal Medicine Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt. 4. d Armed Forces College of Medicine , Cairo , Egypt. 5. e Clinical Oncology Department, Faculty of Medicine , Ain Shams University , Cairo , Egypt.
Abstract
BACKGROUND: Long noncoding RNAs(lncRNAs) have emerged as key elements in modulating gene expression in different biological contexts. PATIENTS AND METHODS: We used quantitative real-time PCR (Qpcr) to evaluate the expression of lncRNA-UCA1 and C-JUN in serum of 70 patients with hepatocellular carcinoma (HCC), 32 patients chronic hepatitis C (CHC) and 38 healthy subjects and their correlation with different clinicopathological factors. RESULTS: The expression of lncRNA-UCA1 and C-JUN was positive in 91.4%HCC patients with strong discriminating power between HCC and healthy subjects and CHC patients as well. The median follow up period was 29 months. The survival analysis showed that both lncRNA-UCA1 and C-JUN were independent prognostic factors. Of note, we identified C-JUN expression changes consistent with the lncRNA-UCA1 target regulation. CONCLUSION: This information sheds light on the possible role of lncRNA-UCA1 and C-JUN mRNA as promising diagnostic and prognostic markers as well as potential therapeutic targets in HCC.
BACKGROUND: Long noncoding RNAs(lncRNAs) have emerged as key elements in modulating gene expression in different biological contexts. PATIENTS AND METHODS: We used quantitative real-time PCR (Qpcr) to evaluate the expression of lncRNA-UCA1 and C-JUN in serum of 70 patients with hepatocellular carcinoma (HCC), 32 patientschronic hepatitis C (CHC) and 38 healthy subjects and their correlation with different clinicopathological factors. RESULTS: The expression of lncRNA-UCA1 and C-JUN was positive in 91.4%HCC patients with strong discriminating power between HCC and healthy subjects and CHCpatients as well. The median follow up period was 29 months. The survival analysis showed that both lncRNA-UCA1 and C-JUN were independent prognostic factors. Of note, we identified C-JUN expression changes consistent with the lncRNA-UCA1 target regulation. CONCLUSION: This information sheds light on the possible role of lncRNA-UCA1 and C-JUN mRNA as promising diagnostic and prognostic markers as well as potential therapeutic targets in HCC.
Authors: Manuela Lanzafame; Gaia Bianco; Luigi M Terracciano; Charlotte K Y Ng; Salvatore Piscuoglio Journal: Int J Mol Sci Date: 2018-02-28 Impact factor: 5.923