The detection for hypochlorite by UV-Vis and fluorescent spectra based on oxidized ring opening and successive hydrolysis reaction.

In this work, two high selective and sensitive fluorescent probes for ClO(-), 7-Hydroxycoumarin and 4-Hydroxycoumarin were designed. The reaction mechanism that we speculated was the oxidized ring opening reaction and hydrolysis. The detection could be realized in quasi-aqueous phase and the detection limits of probe [7] and probe [4] for ClO(-) were found to be 56.8nM and 70.5nM. Furthermore, the probes can be used to cell imagings.