Literature DB >> 27213278

TRIC: Capturing the direct cellular targets of promoter-bound transcriptional activators.

Amanda Dugan1,2, Rachel Pricer1,2, Micah Katz1, Anna K Mapp1,2,3.   

Abstract

Transcriptional activators coordinate the dynamic assembly of multiprotein coactivator complexes required for gene expression to occur. Here we combine the power of in vivo covalent chemical capture with p-benzoyl-L-phenylalanine (Bpa), a genetically incorporated photo-crosslinking amino acid, and chromatin immunoprecipitation (ChIP) to capture the direct protein interactions of the transcriptional activator VP16 with the general transcription factor TBP at the GAL1 promoter in live yeast.
© 2016 The Protein Society.

Entities:  

Keywords:  TATA-binding protein; VP16; covalent chemical capture; cross-linking; transcription factors; unnatural amino acid

Mesh：

Substances：

Year:  2016        PMID： 27213278      PMCID： PMC4972194          DOI： 10.1002/pro.2951

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  27 in total

1.  SAGA is an essential in vivo target of the yeast acidic activator Gal4p.

Authors:  S R Bhaumik; M R Green
Journal:  Genes Dev       Date:  2001-08-01       Impact factor: 11.361

2.  Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA.

Authors:  Joachim Klein; Mark Nolden; Steven L Sanders; Jay Kirchner; P Anthony Weil; Karsten Melcher
Journal:  J Biol Chem       Date:  2002-12-24       Impact factor: 5.157

Review 3.  Chromatin immunoprecipitation assay.

Authors:  Partha M Das; Kavitha Ramachandran; Jane vanWert; Rakesh Singal
Journal:  Biotechniques       Date:  2004-12       Impact factor: 1.993

4.  Reduced binding of TFIID to transcriptionally compromised mutants of VP16.

Authors:  C J Ingles; M Shales; W D Cress; S J Triezenberg; J Greenblatt
Journal:  Nature       Date:  1991-06-13       Impact factor: 49.962

5.  Effects of activation-defective TBP mutations on transcription initiation in yeast.

Authors:  T K Kim; S Hashimoto; R J Kelleher; P M Flanagan; R D Kornberg; M Horikoshi; R G Roeder
Journal:  Nature       Date:  1994-05-19       Impact factor: 49.962

6.  Caught in the act: covalent cross-linking captures activator-coactivator interactions in vivo.

Authors:  Malathy Krishnamurthy; Amanda Dugan; Adaora Nwokoye; Yik-Hong Fung; Jody K Lancia; Chinmay Y Majmudar; Anna K Mapp
Journal:  ACS Chem Biol       Date:  2011-11-14       Impact factor: 5.100

7.  Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator.

Authors:  J L Regier; F Shen; S J Triezenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-01       Impact factor: 11.205

8.  Functional silencing of TATA-binding protein (TBP) by a covalent linkage of the N-terminal domain of TBP-associated factor 1.

Authors:  Tapas K Mal; Shinya Takahata; Sewon Ki; Le Zheng; Tetsuro Kokubo; Mitsuhiko Ikura
Journal:  J Biol Chem       Date:  2007-06-06       Impact factor: 5.157

9.  Transcriptional activation domain of the herpesvirus protein VP16 becomes conformationally constrained upon interaction with basal transcription factors.

Authors:  F Shen; S J Triezenberg; P Hensley; D Porter; J R Knutson
Journal:  J Biol Chem       Date:  1996-03-01       Impact factor: 5.157

10.  Genome-Wide Transcriptional Regulation Mediated by Biochemically Distinct SWI/SNF Complexes.

Authors:  Jesse R Raab; Samuel Resnick; Terry Magnuson
Journal:  PLoS Genet       Date:  2015-12-30       Impact factor: 5.917
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  2 in total

1.  Discovery of Enzymatic Targets of Transcriptional Activators via in Vivo Covalent Chemical Capture.

Authors:  Amanda Dugan; Chinmay Y Majmudar; Rachel Pricer; Sherry Niessen; Jody K Lancia; Hugo Yik-Hong Fung; Benjamin F Cravatt; Anna K Mapp
Journal:  J Am Chem Soc       Date:  2016-09-20       Impact factor: 15.419

2.  Duck Plague Virus pUL48 Protein Activates the Immediate-Early Gene to Initiate the Transcription of the Virus Gene.

Authors:  Tong Zhou; Dengjian Fan; Mingshu Wang; Anchun Cheng; Ying Wu; Qiao Yang; Bin Tian; Renyong Jia; Xumin Ou; Sai Mao; Di Sun; Shaqiu Zhang; Dekang Zhu; Shun Chen; Mafeng Liu; Xin-Xin Zhao; Juan Huang; Qun Gao; Yanling Yu; Ling Zhang
Journal:  Front Microbiol       Date:  2021-12-23       Impact factor: 5.640
  2 in total

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