R Brent Dixon1, Amitava Dasgupta2. 1. Physician's Choice Laboratory Services, Rock Hill, South Carolina, USA. 2. Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, Houston, Texas, USA. Amitava.Dasgupta@uth.tmc.edu.
Abstract
BACKGROUND: DRI cotinine assay is suitable only for screening for cotinine in urine specimens. We studied the reliability of DRI cotinine semiquantitative values by comparing them with the cotinine concentration obtained with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. METHODS: Semiquantitative cotinine concentrations in 39 urine specimens obtained by the DRI immunoassay were compared with cotinine concentrations obtained by LC-MS/MS. RESULTS: The DRI cotinine assay consistently overestimated cotinine values obtained by the LC/MS/MS method (y = 1.1529 x + 252.24, n = 39, R2 = 0.8899) indicating that semiquantitative values obtained using the DRI assay may be unreliable. However, no false-negative results were observed using the DRI assay. CONCLUSION: DRI cotinine assay is suitable only for screening cotinine in urine specimens.
BACKGROUND:DRI cotinine assay is suitable only for screening for cotinine in urine specimens. We studied the reliability of DRI cotinine semiquantitative values by comparing them with the cotinine concentration obtained with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. METHODS: Semiquantitative cotinine concentrations in 39 urine specimens obtained by the DRI immunoassay were compared with cotinine concentrations obtained by LC-MS/MS. RESULTS: The DRI cotinine assay consistently overestimated cotinine values obtained by the LC/MS/MS method (y = 1.1529 x + 252.24, n = 39, R2 = 0.8899) indicating that semiquantitative values obtained using the DRI assay may be unreliable. However, no false-negative results were observed using the DRI assay. CONCLUSION:DRI cotinine assay is suitable only for screening cotinine in urine specimens.
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