L-L Wang1, X-H Zhang, X Zhang, J-K Chu. 1. Clinical Laboratory, the Central Hospital of Yishui, Linyi, Shandong, China. jiankunchu@outlook.com.
Abstract
OBJECTIVE: MiR-30a can target multiple proteins involved in epithelial-to-mesenchymal transition (EMT). In this study, we investigated the association between miR-30a and cisplatin (DDP) sensitivity in gastric cancer. In addition, the regulation of miR-30a in EMT in SGC-7901 cells and SGC-7901/DDP cells and their involvement in cisplatin sensitivity were further investigated. PATIENTS AND METHODS: 20 advanced gastric cancer patients who received platinum-based chemotherapy were recruited. Chemosensitivity was assessed after completion of the therapy. MiR-30a expression was quantified and compared between chemosensitive and chemoresistant groups. SGC-7901 cells and SGC-7901/DDP cells were further used for the in-vitro gain-and-loss study to investigate the effect of miR-30a on EMT and cisplatin sensitivity. RESULTS: Chemosensitive patients had significantly higher miR-30a expression than the chemoresistant counterparts. SGC-7901 cells had significantly higher miR-30a expression than SGC-7901/DDP cells. Knockdown of endogenous miR-30a promoted the elongated fibroblast-like morphologic alteration of SGC-7901 cells and also enhanced Snail and Vimentin expression. MiR-30a overexpression induced morphological changes from an extended, fibroblast-like morphology to more epithelial-like morphology in SGC-7901/DDP cells and decreased Snail and Vimentin level. The cancer cells with miR-30a overexpression had significantly higher DDP sensitivity, while the cells with miR-30a knockdown had decreased sensitivity. CONCLUSIONS: EMT is associated with cisplatin resistance in gastric cancer. MiR-30a is an important miRNA modulating EMT and cisplatin sensitivity of SGC-7901 and SGC-7901/DDP cells.
OBJECTIVE:MiR-30a can target multiple proteins involved in epithelial-to-mesenchymal transition (EMT). In this study, we investigated the association between miR-30a and cisplatin (DDP) sensitivity in gastric cancer. In addition, the regulation of miR-30a in EMT in SGC-7901 cells and SGC-7901/DDP cells and their involvement in cisplatin sensitivity were further investigated. PATIENTS AND METHODS: 20 advanced gastric cancerpatients who received platinum-based chemotherapy were recruited. Chemosensitivity was assessed after completion of the therapy. MiR-30a expression was quantified and compared between chemosensitive and chemoresistant groups. SGC-7901 cells and SGC-7901/DDP cells were further used for the in-vitro gain-and-loss study to investigate the effect of miR-30a on EMT and cisplatin sensitivity. RESULTS: Chemosensitive patients had significantly higher miR-30a expression than the chemoresistant counterparts. SGC-7901 cells had significantly higher miR-30a expression than SGC-7901/DDP cells. Knockdown of endogenous miR-30a promoted the elongated fibroblast-like morphologic alteration of SGC-7901 cells and also enhanced Snail and Vimentin expression. MiR-30a overexpression induced morphological changes from an extended, fibroblast-like morphology to more epithelial-like morphology in SGC-7901/DDP cells and decreased Snail and Vimentin level. The cancer cells with miR-30a overexpression had significantly higher DDP sensitivity, while the cells with miR-30a knockdown had decreased sensitivity. CONCLUSIONS: EMT is associated with cisplatin resistance in gastric cancer. MiR-30a is an important miRNA modulating EMT and cisplatin sensitivity of SGC-7901 and SGC-7901/DDP cells.
Authors: Ling Yuan; Tingting Ma; Wenjing Liu; Yan Chen; Qihui Yuan; Mengyi Ye; Lei Yu; Jiaxin Li; Yang Niu; Yi Nan Journal: Am J Transl Res Date: 2019-10-15 Impact factor: 4.060