| Literature DB >> 27207286 |
Bruno Cogliati1, Michaël Maes2, Isabel Veloso Alves Pereira3, Joost Willebrords2, Tereza Cristina Da Silva3, Sara Crespo Yanguas2, Mathieu Vinken2.
Abstract
Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing, and/or protein expression level. Basically, fluorescent IHC is a short, simple, and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition.Entities:
Keywords: Antibody; Connexins; Fluorophores; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Localization; Microscopy; Morphology
Mesh：
Substances：
Year: 2016 PMID： 27207286 PMCID： PMC4994867 DOI： 10.1007/978-1-4939-3664-9_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745