| Literature DB >> 27207283 |
Michaël Maes1, Joost Willebrords2, Sara Crespo Yanguas2, Bruno Cogliati3, Mathieu Vinken2.
Abstract
Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin RNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis.Entities:
Keywords: Connexins; Minimum Information for publication of Quantitative real-time PCR Experiments; RNA extraction; Reverse transcription
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Year: 2016 PMID： 27207283 PMCID： PMC4994868 DOI： 10.1007/978-1-4939-3664-9_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745