Haibin Zhong1, Ling Cui1, Fan Xu1, Lifei Chen1, Li Jiang1, Hui Huang1, Jiping Xu1, Xin Zhao1, Li Li1, Siming Zeng2, Min Li3. 1. Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China. 2. Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China. eyezsm@163.com. 3. Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, 530021, Nanning, Guangxi, People's Republic of China. eyeminli@163.com.
Abstract
OBJECTIVE: To evaluate the expression and possible roles of Wip1 in retinal astrocytes after optic nerve crush (ONC). METHODS: Expressions of Wip1, GFAP, and p-p65 in ONC model were analyzed by Western blot and immunofluorescence. The mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were analyzed by RT-PCR. RESULTS: Wip1 was up-regulated at 14 days after ONC by Western blot and immunofluorescence. The changes of Wip1 were striking in astrocytes. Furthermore, the protein expression level of p-p65 was paralleled with Wip1 in a time-dependent manner by ONC. In addition, co-localization of Wip1 with Phospho-NF-κB-p65 (p-p65) was detected. Finally, the mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were significantly increased in retina after ONC. CONCLUSIONS: These data were consistent with the hypothesis that Wip1 was implicated in neuroinflammation of retinal astrocytes after ONC via NF-κB signaling pathway.
OBJECTIVE: To evaluate the expression and possible roles of Wip1 in retinal astrocytes after optic nerve crush (ONC). METHODS: Expressions of Wip1, GFAP, and p-p65 in ONC model were analyzed by Western blot and immunofluorescence. The mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were analyzed by RT-PCR. RESULTS:Wip1 was up-regulated at 14 days after ONC by Western blot and immunofluorescence. The changes of Wip1 were striking in astrocytes. Furthermore, the protein expression level of p-p65 was paralleled with Wip1 in a time-dependent manner by ONC. In addition, co-localization of Wip1 with Phospho-NF-κB-p65 (p-p65) was detected. Finally, the mRNA expressions of the pro-inflammatory cytokines (IL-8, TNF-α, IL-6 and IL-1β) were significantly increased in retina after ONC. CONCLUSIONS: These data were consistent with the hypothesis that Wip1 was implicated in neuroinflammation of retinal astrocytes after ONC via NF-κB signaling pathway.
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