Literature DB >> 271974

New method for localizing proteins in periodic structures: Fab fragment labeling combined with image processing of electron micrographs.

U Aebi, B ten Heggeler, L Onorato, J Kistler, M K Showe.   

Abstract

Fab fragments prepared from antisera directed against purified bacteriophage T4 structural proteins and head-related structures were used to label proteins on the surface of T-even giant phage capsids. Optically filtered electron micrographs of the Fab-labeled capsids reveal both the location of specific proteins within the capsomeres and differing conformational states of the protein subunits. We describe parameters affecting the utility of this technique for the study of molecular organization and protein conformation in periodic biological structures.

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Year:  1977        PMID： 271974      PMCID： PMC431787          DOI： 10.1073/pnas.74.12.5514

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.

Authors:  R R PORTER
Journal:  Biochem J       Date:  1959-09       Impact factor: 3.857

2.  Bacteriophage T4 prehead proteinase. II. Its cleavage from the product of gene 21 and regulation in phage-infected cells.

Authors:  M K Showe; E Isobe; L Onorato
Journal:  J Mol Biol       Date:  1976-10-15       Impact factor: 5.469

3.  Comparison of the structural and chemical composition of giant T-even phage heads.

Authors:  U Aebi; R K Bijlenga; B ten Heggeler; J Kistler; A C Steven; P R Smith
Journal:  J Supramol Struct       Date:  1976

4.  Structure of T4 polyheads. II. A pathway of polyhead transformation as a model for T4 capsid maturation.

Authors:  A C Steven; E Couture; U Aebi; M K Showe
Journal:  J Mol Biol       Date:  1976-09-05       Impact factor: 5.469

5.  Structural aberrations in T-even bacteriophage. 3. Induction of "lollipops" and their partial characterization.

Authors:  D J Cummings; V A Chapman; S S DeLong; N L Couse
Journal:  Virology       Date:  1973-07       Impact factor: 3.616

6.  Structure of the distal half of the bacteriophage T4 tail fiber.

Authors:  S K Beckendorf
Journal:  J Mol Biol       Date:  1973-01       Impact factor: 5.469

7.  Genetic control of capsid length in bacteriophage T4. I. Isolation and preliminary description of four new mutants.

Authors:  A H Doermann; F A Eiserling; L Boehner
Journal:  J Virol       Date:  1973-08       Impact factor: 5.103

8.  Molecular organization of the shell of T-even bacteriophage head. II. Arrangement of subunits in the head shell of giant phages.

Authors:  M Yanagida
Journal:  J Mol Biol       Date:  1977-02-05       Impact factor: 5.469

9.  Capsid fine structure of T-even bacteriophages. Binding and localization of two dispensable capsid proteins into the P23* surface lattice.

Authors:  U Aebi; R van Driel; R K Bijlenga; B ten Heggeler; R van den Broek; A C Steven; P R Smith
Journal:  J Mol Biol       Date:  1977-03-15       Impact factor: 5.469

10.  Freeze drying and shadowing a two-dimensional periodic specimen.

Authors:  J Kistler; U Aebi; E Kellenberger
Journal:  J Ultrastruct Res       Date:  1977-04
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  8 in total

1.  Cryo-EM study of Hepatitis B virus core antigen capsids decorated with antibodies from a human patient.

Authors:  Eaazhisai Kandiah; Norman R Watts; Naiqian Cheng; Giovanni Cardone; Stephen J Stahl; Theo Heller; T Jake Liang; Paul T Wingfield; Alasdair C Steven
Journal:  J Struct Biol       Date:  2011-10-25       Impact factor: 2.867

2.  Molecular mechanisms controlling protein-protein and protein-nucleic acid interactions as revealed by studies of virus maturation.

Authors:  E Kellenberger
Journal:  Experientia       Date:  1980-03-15

3.  Fabs enable single particle cryoEM studies of small proteins.

Authors:  Shenping Wu; Agustin Avila-Sakar; JungMin Kim; David S Booth; Charles H Greenberg; Andrea Rossi; Maofu Liao; Xueming Li; Akram Alian; Sarah L Griner; Narinobu Juge; Yadong Yu; Claudia M Mergel; Javier Chaparro-Riggers; Pavel Strop; Robert Tampé; Robert H Edwards; Robert M Stroud; Charles S Craik; Yifan Cheng
Journal:  Structure       Date:  2012-04-03       Impact factor: 5.006

4.  Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis.

Authors:  J Smit; D A Grano; R M Glaeser; N Agabian
Journal:  J Bacteriol       Date:  1981-06       Impact factor: 3.490

5.  Distinct monoclonal antibodies separately label the hexons or the pentons of herpes simplex virus capsid.

Authors:  B L Trus; W W Newcomb; F P Booy; J C Brown; A C Steven
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-01       Impact factor: 11.205

6.  A nonadjuvanted polypeptide nanoparticle vaccine confers long-lasting protection against rodent malaria.

Authors:  Stephen A Kaba; Clara Brando; Qin Guo; Christian Mittelholzer; Senthilkumar Raman; David Tropel; Ueli Aebi; Peter Burkhard; David E Lanar
Journal:  J Immunol       Date:  2009-11-13       Impact factor: 5.422

7.  Optimizing the refolding conditions of self-assembling polypeptide nanoparticles that serve as repetitive antigen display systems.

Authors:  Yongkun Yang; Philippe Ringler; Shirley A Müller; Peter Burkhard
Journal:  J Struct Biol       Date:  2011-11-17       Impact factor: 2.867

8.  Identification of recombinant Fabs for structural and functional characterization of HIV-host factor complexes.

Authors:  Natalia Sevillano; Evan M Green; Jörg Votteler; Dong Young Kim; Xuefeng Ren; Bei Yang; Xi Liu; André Luiz Lourenço; James H Hurley; Shauna Farr-Jones; John D Gross; Yifan Cheng; Charles S Craik
Journal:  PLoS One       Date:  2021-05-13       Impact factor: 3.240
  8 in total

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