Literature DB >> 27196638

Development of a real-time PCR assay for quantification of Citrobacter rodentium.

Sofia Sagaidak1, Amel Taibi1, Bijun Wen1, Elena M Comelli2.   

Abstract

Molecular tools to quantify Citrobacter rodentium are not available. We developed a quantitative PCR assay targeting the espB gene. This assay is specific, has a linearity range of about 6.7×10(1) to 6.7×10(6)cells/PCR reaction (92% efficiency) and a detection limit of about 10(4)cells/g wet feces.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Citrobacter rodentium; Mice; espB; qPCR

Mesh：

Substances：

Year:  2016        PMID： 27196638     DOI： 10.1016/j.mimet.2016.05.008

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  8 in total

1.  PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.

Authors:  Susan R Compton
Journal:  J Am Assoc Lab Anim Sci       Date:  2020-06-24       Impact factor: 1.232

2.  Human gut bacteria produce ΤΗ17-modulating bile acid metabolites.

Authors:  Donggi Paik; Lina Yao; Yancong Zhang; Sena Bae; Gabriel D D'Agostino; Minghao Zhang; Eunha Kim; Eric A Franzosa; Julian Avila-Pacheco; Jordan E Bisanz; Christopher K Rakowski; Hera Vlamakis; Ramnik J Xavier; Peter J Turnbaugh; Randy S Longman; Michael R Krout; Clary B Clish; Fraydoon Rastinejad; Curtis Huttenhower; Jun R Huh; A Sloan Devlin
Journal:  Nature       Date:  2022-03-16       Impact factor: 69.504

3.  The neonatal microenvironment programs innate γδ T cells through the transcription factor STAT5.

Authors:  Darshana Kadekar; Rasmus Agerholm; John Rizk; Heidi A Neubauer; Tobias Suske; Barbara Maurer; Monica Torrellas Viñals; Elena M Comelli; Amel Taibi; Richard Moriggl; Vasileios Bekiaris
Journal:  J Clin Invest       Date:  2020-05-01       Impact factor: 14.808

4.  Intestinal IL-17R Signaling Controls Secretory IgA and Oxidase Balance in Citrobacter rodentium Infection.

Authors:  Yasuka Matsunaga; Trevon Clark; Alanna G Wanek; Jacob P Bitoun; Qingqing Gong; Misty Good; Jay K Kolls
Journal:  J Immunol       Date:  2021-01-11       Impact factor: 5.422

5.  Maternal gut bacteria drive intestinal inflammation in offspring with neurodevelopmental disorders by altering the chromatin landscape of CD4+ T cells.

Authors:  Eunha Kim; Donggi Paik; Ricardo N Ramirez; Delaney G Biggs; Youngjun Park; Ho-Keun Kwon; Gloria B Choi; Jun R Huh
Journal:  Immunity       Date:  2021-12-07       Impact factor: 31.745

6.  Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis.

Authors:  Zhi Cheng Wu; Hong Xin Feng; Lin Wu; Meng Zhang; Wei Lan Zhou
Journal:  ACS Omega       Date:  2020-10-13

7.  Pectins that Structurally Differ in the Distribution of Methyl-Esters Attenuate Citrobacter rodentium-Induced Colitis.

Authors:  Martin Beukema; Renate Akkerman; Éva Jermendi; Taco Koster; Anne Laskewitz; Chunli Kong; Henk A Schols; Marijke M Faas; Paul de Vos
Journal:  Mol Nutr Food Res       Date:  2021-08-16       Impact factor: 6.575

8.  Cathelicidin-mediated lipopolysaccharide signaling via intracellular TLR4 in colonic epithelial cells evokes CXCL8 production.

Authors:  Ravi Holani; Anshu Babbar; Graham A D Blyth; Fernando Lopes; Humberto Jijon; Derek M McKay; Morley D Hollenberg; Eduardo R Cobo
Journal:  Gut Microbes       Date:  2020-07-13
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.