Supplementation of Pork Patties with Bovine Plasma Protein Hydrolysates Augments Antioxidant Properties and Improves Quality.

This study investigated the effects of bovine plasma protein (PP) hydrolysates on the antioxidant and quality properties of pork patties during storage. Pork patties were divided into 4 groups: without butylated hydroxytoluene (BHT) and PP hydrolysates (control), 0.02% BHT (T1), 1% PP hydrolysates (T2), and 2% PP hydrolysates (T3). Pork patty supplemented with PP hydrolysates had higher pH values and lower weight loss during cooking than the control patties. Results showed that lightness and hardness both decreased upon the addition of PP hydrolysates. All samples containing BHT and PP hydrolysates had reduced TBARS and peroxide values during storage. In particular, 2% PP hydrolysates were more effective in delaying lipid oxidation than were the other treatments. It was concluded that treatment with 2% PP hydrolysates can enhance the acceptance of pork patty.

Introduction

Lipid oxidation is a key factor in the deterioration of meat and meat products during processing and storage, leading to undesirable changes in color, flavor, texture, and nutritional profile. The extent and speed of oxidation depends on several factors, such as temperature, the presence of prooxidants and antioxidants, and the molecular nature of the lipids in the products (Frankel, 1985; Park ). Antioxidants have been widely used to prevent the oxidative process in meat and meat products (Jung ). The use of antioxidants is an effective method of preventing the generation of lipid oxidation products while maintaining nutritional quality and extending shelf-life.

The most common synthetic antioxidants used in the meat industry are butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Both BHA and BHT have been widely used for many years to delay lipid oxidation and extend the shelf-life of meat and meat products (Nuñez de Gonzalez ). However, concerns about the long-term safety and negative consumer perception of synthetic antioxidants have led to an increasing demand for the use of natural antioxidants in meat and meat products (Ahn ). It has been reported that some protein and enzymatic hydrolysates of meat and meat by-products are able to exert antioxidant activity in food products (Li ). In line with this, the demand for peptides or proteins as antioxidants in foods is increasing due to their low cost, safety, and high nutritional values (Hattori ).

In recent years, research has focused on the generation of peptides from food sources (Daoud ), which have been shown to exert antimicrobial, antihypertensive, and antioxidant effects. Peptides with antioxidative activity have been identified from enzymatic hydrolysates of egg albumin (Tsuge ), casein (Rival ), whey protein (Peña-Ramos ), gelatin (Kim ), and myofibrillar protein (Saiga ).

Plasma protein can be used in both the feed and food industries owing to its good nutritional value and excellent functional properties (Tybor ). Plasma protein hydrolysates, such as porcine plasma (Liu ) and bovine plasma (Salgado ), have been shown to possess antioxidant activity. However, hydrolyzed plasma protein has not been used as a functional food ingredient nor has it been evaluated as a potential antioxidant for food quality preservation. Also, there is little information regarding the functional peptides generated and how to establish antioxidant activity in meat-based by-products such as bovine plasma protein.

The objective of the present study was to determine the effectiveness of bovine plasma protein hydrolysates in preventing lipid oxidation in fresh pork patty. Also, the influence of the hydrolyzed plasma protein on other quality traits of the pork patties such as their color, texture, and sensory properties was investigated.

Materials and Methods

Preparation of bovine plasma protein hydrolysates

The plasma protein (PP) hydrolysates were prepared according to the method described by Liu Cattle blood was anticoagulated by adding 0.5 N ethylenediaminetetraacetic acid (EDTA) in a 1:9 (v/v) proportion. This blood was immediately placed on ice and transported to the laboratory within 30 min. Samples were centrifuged (SUPRA 25K, Hanil Science, Korea) at 14,000 g for 15 min at 4℃. The plasma was then freeze-dried (Clean van 8B Freeze-Dryer, BioTron, Inc., Korea), pulverised, and stored in sealed bags at 4℃.

Bovine PP solution [5% w/v 10 mM sodium phosphate buffer (pH 7.0)] was heat treated (90℃, 5 min) and then hydrolyzed with Alcalase, with an enzyme to substrate ratio (E/S) of 2:100 (g/g). The pH of the bovine PP solution was adjusted to the optimal value for Alcalase (pH 8.32) before hydrolysis was initiated, and it was readjusted to the optimal value every 15 min during hydrolysis with 1 M NaOH. The hydrolysates were produced by setting the hydrolyzation time to 338 min and the temperature to 54℃. After hydrolysis, the pH of the solution was brought to 7.0 and the solution was then heated at 95℃ for 5 min to inactivate the enzyme.

The degree of hydrolysis (DH) was determined by assaying free amino groups with 2, 4, 6-trinitrobenzenesulfonic acid (TNBS) according to Alder-Nissen (1986). The free amino content in samples was expressed as leucine amino equivalents, based on the equation of the leucine standard curve generated. The DH of hydrolyzed PP was calculated as [(h − h0) / (h − h0)] × 100 (DH = 18.8%, respectively).

Here h and h0 represent respectively the amino concentrations of hydrolyzed and non-hydrolyzed PP and h represents the total amino concentration of PP, as measured by completely hydrolyzing the PP with 6 N HCl. The hydrolysates were freeze-dried (Clean van 8B Freeze-Dryer, BioTron, Inc., Korea), pulverized, and stored in sealed bags at 4℃.

Preparation of pork patty

A total of nine pigs (Landrace × Yorkshire × Duroc; 100±5 kg) were randomly selected at a commercial slaughter plant. Pork longissimus dorsi muscles (pH 5.54-5.57) were obtained at 48 h post-slaughter from a local market on three different processing days. Three replications of six pork loins (3 pigs) per replicate were used for each treatment. Each loin was trimmed of any visible fat and connective tissues, and each replication was ground separately through a 3-mm plate, twice. In each replication, four pork patty formulations, all with 1.5% (w/w) added NaCl, were prepared: (C) control (without the addition of BHT and PP hydrolysates); (T1) 0.02% BHT; (T2) 1% PP hydrolysates; (T3) 2% PP hydrolysates. For each formulation treatment, the mixture was prepared by blending for 5 min with a Kitchen Aid mixer (5K5SS, Kitchen Aid, USA). Duplicate patties of 100 g each were shaped by hand into approximately 10 cm (diameter) × 1 cm (thickness) rounds. The patties were placed in styrene foam trays and wrapped in an oxygen permeable poly (vinyl chloride) film, stored at 4℃ for 1, 4 and 7 d of storage.

The rest of the patties were cooked in an electric oven at 175℃ to an internal temperature of 75℃ and used for sensory analysis. All cooked patties were vacuum-packaged immediately after cooking to minimize oxidative changes during handling before the sensory test. The cooked meats were tested for sensory test without storage.

Proximate analysis

The moisture (method 920.36), crude protein (method 984.13), crude fat (method 991.36), and crude ash (method 938.08) contents were determined according to Association of Official Analytical Chemists (AOAC, 2000).

pH measurement

The pH was measured in triplicate using a digital pH meter (MP230, Mettler Toledo, Switzerland). Approximately 3 g of patty sample was added to distilled water (27 mL). A slurry was then made using a homogenizer (ULTRA TURRAX T25D, IKA, Germany) and the pH was measured. The pH meter was calibrated daily with standard buffers of pH 4.0 and 7.0 at 25℃.

Cooking loss

Pork patties were cooked on a heated metal plate (170℃) by flipping every 2 min until the patties’ internal temperature reached 73℃. The weight loss due to cooking was determined for each treatment replication combination. Weights of uncooked and cooked patties were recorded (Boles and Swan, 1996). The cooking loss was calculated as follows:

Cooking loss (%) = cooked weight / uncooked weight × 100

Color

Color was measured using a Labscan Spectrophotometer (Hunter Associates Laboratory, Inc., USA) that had been calibrated against white and black reference tiles covered with the same film as those used for patty samples. CIE L* (Lightness), a* (redness), b* (yellowness) values were obtained using illuminant A (light source). Area view and port size were 0.64 and 1.02 cm, and observer angle was 10°. An average value from three random locations of sample surface was used for statistical analysis.

Texture profile analysis (TPA)

TPA was performed using an Instron University Testing Machine (Model 3343) with a 49 N load cell. Prior to testing, cooked pork patties were equilibrated to room temperature for 30 min and were cut into 2.5 cm diameter samples. The samples were compressed twice at a crosshead speed of 100 mm/min to 70% of their original height using a 3-in. diameter cylindrical plate. The textural parameters of hardness, cohesiveness, springiness, and gumminess were calculated.

2-thiobarbituric acid-reactive substance (TBARS)

Lipid oxidation was determined using a TBARS method (Buege and Aust, 1978). The amounts of TBARS were expressed as mg of malondialdehyde (MDA) per kg of sample.

Peroxide values (PV)

The PV of pork patties were measured according to the AOCS standard procedure (1993). Specifically, patties were finely chopped by blending in a microwaving blender for exactly 30 s. A 5 g sample was then mixed with 25 mL of acetic acid-chloroform solution (3:2). The slurry was gently swirled to extract lipid and 1 mL saturated potassium iodine solution was then added. After reaction for 1 min with occasional shaking, 30 mL of distilled H2O and 1 mL of 0.1% starch solution were added. The solution was titrated with 0.01 N sodium thiosulfate (Na2S2O3) until the intense blue color disappeared. A control blank (without meat sample) was also analyzed. The PV was calculated as

PV (mequiv/kg) = [(S − B) × 1000 × N] / W

where S and B are the volume (mL) of sodium thiosulfate solution consumed by the sample and by the blank, respectively, N is the concentration (N) of sodium thiosulfate solution, and W is the sample weight (g).

Sensory evaluation

Pork patty samples from each treatment were evaluated by an 8-member trained expert descriptive attribute sensory panel in the Gyeongsang National University. Recruitment, selection, and training of panelists were performed according to the sensory evaluation procedure (Meilgaard, 1999). Eight panelists were screened from 12 potential panelists using a basic taste identification test and were trained with commercial pork patty products for 2 wk to familiarize them with the product characteristics planned to evaluated. Panelists were given samples representing anchor points for each attribute, and training sessions using pork patties without antioxidant (control) and pork patties with antioxidant (BHT and 1%, 2% PP). The panelists were trained using a 5-point scale (“5 extremely intense” and “1 slightly intense”) for color, cooked pork flavor, antioxidant flavor and texture attributes (hardness and juiciness).

Panelists evaluated the color, flavor, off-flavor, juiciness, tenderness, and overall acceptability of the samples using a 9-point hedonic, where 1 was “dislike extremely” and 9 was “like extremely” as described by Meilgaard . Four samples were provided to each panelist per session (3 sessions per each treatment). The samples were placed in glass containers (Pyrex, Belgium) with plastic covers before the sensory test.

Statistical analysis

The experiment had three replications. Data was analyzed by the procedures of generalized linear model (GLM) of SAS (2014) Duncan’s multiple range test was used to compare the mean values of treatments. Mean values and standard error of the means (SEM) were reported. Differences in sensory values were compared using the Tukey’s significant differences. For sensory data, mean values and standard deviations were reported. Statistical significance for all comparisons was made at p<0.05.

Results and Discussion

The proximate compositions of the pork patties in each group are shown in Table 1. The T1, T2, and T3 pork patties showed no significant difference in crude protein and crude fat contents compared to the control patties. However, the moisture content of the pork patty samples ranged from 62.18% to 64.52%, with The T3 patties showing a higher moisture content than the patties in the other treatment groups (p<0.05). This finding suggests that the evaluation of physical properties and sensory quality in the T3 patties could potentially be biased by different moisture contents. It was also found that the ash content of pork patties was increased by the addition of BHT and hydrolyzed PP (p<0.05).

Table 1.

Proximate composition (%) of pork patties

Treatments1)	Moisture	Crude protein	Crude fat	Ash
C	63.14B	20.42	10.61	1.33B
T1	62.81B	21.77	10.70	1.67A
T2	63.21B	20.98	11.18	1.65A
T3	64.52A	20.16	10.62	1.76A
SEM	0.62	0.03	0.50	0.01

SEM: standard error of the means (n=3).

A,BMeans differ significantly (p<0.05) between treatment groups within a column.

1C, control without the addition of BHT or protein hydrolysates; T1, 0.02% BHT; T2, 1% plasma protein hydrolysates; T3, 2% plasma protein hydrolysates.

pH and cooking loss

Changes in pH and cooking weight loss in pork patties during storage at 4℃ are presented in Table 2. During storage, the control and T1 pork patty did not show significant changes in pH during 7 d of storage. The T2 patties had a lower pH at the start, but this increased after 4 d and then maintained by the end of storage (p<0.05). The T3 patties had the highest pH values throughout the storage period. Thus, in comparison to the control patties, the patties containing PP hydrolysates had higher pH values over the 7 d storage period (p<0.05). The relationship between pH and muscle protein denaturation has previously been reported by Bendall and Wismer-Pederson (1962). Further, the effectiveness of antioxidants is dependent on pH. It was previously shown that the pH value of chicken meat was increased with garlic supplementation (Kim ). Thus, similar to previous studies, the present experiment has shown that the pH value of pork patty can be altered through the addition of hydrolyzed PP.

Table 2.

pH, cooking weight loss (%), and color (CIE values) in pork patties during storage at 4℃

Treatments1)		Storage periods (d)
		1	4	7	SEM
pH	C	5.75B	5.65C	5.65C	0.01
	T1	5.62C	5.62C	5.62C	0.00
	T2	5.60Cc	5.75Ba	5.71Bb	0.00
	T3	5.85Aab	5.87Aa	5.81Ab	0.00
	SEM	0.01	0.01	0.00
Cooking loss (%)	C	23.78Aa	23.61Aa	20.98Ab	0.81
	T1	21.51B	22.59A	21.23A	1.50
	T2	15.41C	16.77B	15.52B	0.95
	T3	11.30D	12.22C	11.67C	0.63
	SEM	1.26	0.78	1.03
L* (Lightness)	C	56.06A	57.17B	56.82AB	5.30
	T1	56.95Ab	59.17Aa	57.29Ab	4.56
	T2	54.17Bb	56.58BCa	57.51Aa	4.89
	T3	52.85Bb	55.57Ca	55.72Ba	6.15
	SEM	5.04	5.25	5.40
a* (Redness)	C	12.17Aa	11.52Aab	10.92Ab	2.43
	T1	12.27Aa	11.34Ab	11.07Ab	1.13
	T2	11.90Aa	10.60Bb	9.36Bc	0.94
	T3	10.83Ba	9.89Cb	9.54Bb	0.34
	SEM	2.02	0.81	0.81
b* (Yellowness)	C	5.75D	5.64C	5.59C	0.83
	T1	6.38Ca	5.63Cb	5.66Cb	0.64
	T2	7.51Ba	7.03Bab	6.83Bb	0.63
	T3	8.33A	8.39A	8.53A	0.67
	SEM	0.73	0.84	0.51

SEM: standard error of the means (n=3).

A-DMeans differ significantly (p<0.05) between treatment groups within a column.

a,bMeans differ significantly (p<0.05) between storage times within a row.

1C, control without the addition of BHT or protein hydrolysates; T1, 0.02% BHT; T2, 1% plasma protein hydrolysates; T3, 2% plasma protein hydrolysates.

The weight loss during cooking was significantly reduced in the T1, T2, and T3 patties compared with the control patties over the 7 d storage period (p<0.05). Hydrolyzed PP was particularly effective, reducing the weight loss of control patties (23.78%) to 15.41% and 11.30% (p<0.05) in T2 and T3 patties, respectively. Furthermore, higher concentrations of added PP hydrolysates tended to decrease percent cooking loss. This variation in weight loss during cooking of pork patties was expected as a positive relationship between weight loss and muscle pH has been previously demonstrated (Joo ). In line, an increase in pH in the antioxidant-treated samples was associated with reduced weight loss during cooking in the present study. Consistently, Peña-Ramos and Xiong (2003) also reported a reduction in weight loss during cooking in ground meat supplemented with soy and whey protein hydrolysates. A potential explanation for this relationship was suggested in a previous study, in which it was found that hydrolysis led to dissociation of potato protein into subunits, thus producing additional polar and charged groups allowing for stronger protein-water interactions in meat (Wang and Xiong, 2005).

The changes in color pork patties during storage at 4℃ are presented in Table 2. During storage, the control pork patty did not show significant changes in lightness (L*) during 7 d of storage. However, the L* vales increased during storage for 7 d in treatment groups. On day 1, T2 and T3 pork patties had lower L* values than the patties in the other treatment groups (p<0.05), with the T3 patties showing the lowest L* values across the 7 d storage period. Antioxidants such as α-tocopherol and rosemary are effective in preventing meat discoloration. It is widely accepted that variations in muscle structure may affect the extent of denaturation of muscle protein, allowing for differences between pale and dark colored meats (Chen ). Further, the different results may be related to the different materials or processing methods employed. Beggs have shown that the level of modified starch added to turkey frankfurters affected color values. Additionally, Nuñez de Gonzalez reported that the color of pork sausage supplemented with dried plum is darker due to the original dark purple color of the plum.

Redness (a*) varied from 9.36 to 12.27 over the storage time period. All pork patty samples showed significant changes in a* values during 7 d of storage at 4℃. Redness values were lower in the T2 and T3 pork patties compared to the control and T1 patties throughout the 7 d period (p<0.05). Yellowness (b*) values were higher in the T2 and T3 patties compared to the control and T1 patties, with the T3 patties showing the highest values across the 7 d storage period (p<0.05). The b* values of the T2 and T3 pork patties were slightly higher compared to that of the T1 patties. Thus, the addition of PP hydrolysates changed the color attributes of the pork patties by decreasing lightness and redness and increasing yellowness.

Texture profile analysis

The texture attributes of the pork patties are shown in Table 3. Hardness values were tended to lower for the T2 and T3 patties compared to the patties in the other treatment groups (p<0.05). Furthermore, on day 4, the patties containing PP hydrolysates (T2 and T3) showed significantly decreased hardness values compared to the patties in the control and T1 groups (p<0.05). Also, the cohesiveness and springiness values were higher in the T3 patties compared to the patties in the other treatment groups throughout storage, expect at 4 d (p<0.05). All treatments (T1, T2, and T3) had lower gumminess values over the 7 d storage period. Of the treated samples, gumminess values were lower in the T2 patties compared to the patties in the other treatment group over the 7 d period (p<0.05).

Table 3.

Texture properties of pork patties during storage at 4℃

Treatments1)		Storage periods (d)
		1	4	7	SEM
Hardness (N)	C	4.28Ab	5.96Aa	4.50Ab	0.88
	T1	3.42ABb	5.06Aa	3.88ABb	0.58
	T2	3.00B	3.07B	3.60B	0.36
	T3	3.78Aa	2.27Bb	4.15ABa	0.54
	SEM	0.40	0.11	0.35
Cohesiveness	C	0.36Bb	0.47a	0.46Bab	0.04
	T1	0.38Bb	0.45a	0.44Ba	0.01
	T2	0.41Bb	0.45ab	0.49Ba	0.03
	T3	0.56A	0.50	0.60A	0.07
	SEM	0.03	0.02	0.03
Springiness	C	26.22B	21.57	17.75B	3.01
	T1	13.79Cb	22.28a	16.52Bab	3.47
	T2	20.86BC	21.61	27.17B	1.03
	T3	50.81Aa	20.75b	53.64Aa	4.05
	SEM	2.60	3.70	3.04
Gumminess (N)	C	24.50ABc	41.89Aa	35.62Ab	1.70
	T1	22.84BCc	36.27Aa	29.35BCb	1.19
	T2	19.60Cb	23.21Bab	25.59Ca	1.53
	T3	26.20Ab	18.80Bc	33.33ABa	1.22
	SEM	0.73	2.48	1.17

SEM: standard error of the means (n=3).

A-CMeans differ significantly (p<0.05) between treatment groups within a column.

a-cMeans differ significantly (p<0.05) between storage times within a row.

1C, control without the addition of BHT or protein hydrolysates; T1, 0.02% BHT; T2, 1% plasma protein hydrolysates; T3, 2% plasma protein hydrolysates.

These results indicate that the addition of PP hydrolysates is useful in preparing a pork patty with softer textural properties. These results are consistent with those reported by other researchers, who demonstrated that the addition of hydrolyzed soy protein isolates improved textural properties by decreasing product hardness (Feng ). Yang suggested that a decrease in the hardness of sausage by the addition of texture-modifying agents may be associated with the water binding properties of the ingredients.

Inhibition of lipid oxidation in pork patties

The results of the lipid oxidation analysis of pork patties during storage at 4℃ are shown in Figs. 1 and 2. The TBARS assay is commonly used for detecting the decomposition products of peroxides, such as aldehydes (Angelo, 1996). In all treatment groups, the pork patties showed an increasing trend in TBARS as the storage period increased (Fig. 1). However, TBARS values were lower in the T2 and T3 patties compared to the patties in the other treatment groups throughout the 7 d period (p<0.05). Overall, both BHT and hydrolyzed PP inhibited TBARS formation in patties during storage. In particular, the addition of PP hydrolysates was more effective in decreasing TBARS values of the pork patties than was the addition of BHT after 4 d storage (p<0.05).

Fig. 1.

TBARS values in pork patties during storage period at 4℃. A-CMeans differ significantly (p<0.05) between treatment groups. a-cMeans differ significantly (p<0.05) between storage times within a treatment group. Control, without the addition of BHT or protein hydrolysates; T1, 0.02% BHT; T2, 1% plasma protein hydrolysates; T3, 2% plasma protein hydrolysates.

Fig. 2.

Peroxide value (PV) of pork patties during storage at 4℃. A-CMeans differ significantly (p<0.05) between treatment groups. a-cMeans differ significantly (p<0.05) between storage times within a treatment group. Control, without the addition of BHT or protein hydrolysates; T1, 0.02% BHT; T2, 1% plasma protein hydrolysates; T3, 2% plasma protein hydrolysates.

The peroxide value (PV) is used as a measure of primary oxidation products in meat and meat products. The progress of lipid oxidation as indicated by PV following the addition of BHT and PP hydrolysates to pork patties is shown in Fig. 2. In all treatment groups, the PV at day 7 had dramatically increased compared to day 1 (p<0.05). However, the PV was lower in the T1, T2, and T3 pork patties compared to the control patties throughout the 7 d period (p<0.05). Furthermore, the addition of PP hydrolysates was more effective in decreasing PV of pork patties than was the addition of BHT during storage (p<0.05). Also, the PV of pork patties treated with 1 or 2% hydrolyzed plasma protein (2.46 and 2.01 mequiv/kg) at the end of storage represented 32.78% and 45.08% oxidation inhibitions (p<0.05), respectively, when compared with that of control patties (3.66 mequiv/kg).

The antioxidative effect of plasma protein hydrolysates on pork patties in this study was remarkable. In concordance, Wang and Xiong (2005) reported that hydrolyzed potato proteins inhibited PV and TBARS formation in cooked beef patties during storage. Additionally, several previous studies have indicated that hydrolyzed whey and soy protein (Peña-Ramos and Xiong, 2003) mechanically deboned chicken (Jin ) egg-yolk protein (Sakanaka et al., 2004), and porcine haemoglobin (Chang ) were antioxidative in meat products. Therefore, adding PP hydrolysates to fresh pork patties affords enhanced antioxidant activity and protection against lipid oxidation.

Ultimately, meat product quality is defined in terms of consumer acceptability which includes color, tenderness, juiciness, tenderness and flavor (Robbins ). In addition, appearance characteristics have a significant impact on consumer expectations (Brewer and Novakofski, 2008). The results of the sensory evaluation of pork patties are shown in Table 4. There were no significant differences in flavor and texture scores (p>0.05) between any of the treatment groups. The off-flavor scores in pork patties with added BHT and PP hydrolysates were higher than those in the control patties (p<0.05). Addition of crude wheat gluten hydrolysate in pork patties did not adversely affect the color, smell, taste, texture, and overall acceptability of cooked pork (Park ). Further, increasing levels of PP hydrolysates tended to increase the off-flavor scores. However, sensory scores for color and juiciness were higher in the patties with added PP hydrolysates than in the control patties. Some authors suggested that juiciness scores increased primarily as a result of increased moisture (Gök ; Yi ). The addition of 2% PP hydrolysates resulted in significantly higher color and juiciness scores compared to all other treatments (p<0.05). The overall acceptability scores ranged from 4.82 to 6.22, with maximum acceptability obtained in the patties containing 2% PP hydrolysates.

Table 4.

Sensory evaluations of pork patties (Tukey’s HSD 5%)

Treatments1)	Color	Flavor	Off-flavor	Juiciness	Texture	Overall acceptability
C	3.75±0.97B	4.92±1.16	2.17±0.58C	4.33±1.30C	4.58±1.16	4.82±0.75B
T1	4.27±0.90AB	4.91±1.45	2.27±0.79BC	4.73±1.42C	4.64±1.12	5.00±1.05B
T2	4.55±1.04AB	5.00±1.48	2.82±0.75AB	5.64±1.30AB	4.82±0.98	5.70±0.95AB
T3	5.00±1.49A	4.90±1.45	3.20±0.63A	6.10±0.88A	5.00±1.15	6.22±1.30A

Data are means±standard errors.

A-CMeans differ significantly (p<0.05) between treatment groups within a column. (n=8).

1C, control without the addition of BHT or protein hydrolysates; T1, 0.02% BHT; T2, 1% plasma protein hydrolysates; T3, 2% plasma protein hydrolysates.

Based on a 9-point intensity scale (1 = dislike extremely or extremely light/bland/tough/dry; and 9 = like extremely or extremely dark/intense/tender/juice.

Conclusion

This study concluded that PP hydrolysates provide antioxidant and quality benefits to fresh pork patties during refrigerated storage. Pork patties supplemented with PP hydrolysates have higher pH, and lower hardness and lightness values than control patties. Hydrolyzed PP products at the 1 or 2% level were able to not only reduce the weight loss during cooking but also to suppress lipid oxidation in pork patties during refrigerated storage. The addition of PP hydrolysates was more effective than the addition of BHT in decreasing peroxide and TBARS values of pork patties during storage. These results indicate that the PP hydrolysates were as effective as, if not superior to, BHT in retarding lipid oxidation in pork patty. Furthermore, the addition of 2% PP hydrolysates resulted in significantly higher color, juiciness, and overall acceptability scores compared to all other treatments (p<0.05). Overall, the combination of the antioxidant effect together with the enhanced quality properties highlight the potential for PP hydrolysates to be utilized as attractive natural ingredients in processed muscle foods.