AIM: To analyze the lipid distribution in gastric mucosae. METHODS: Imaging mass spectrometry (MS) is a useful tool to survey the distribution of biomolecules in surgical specimens. Here we used the imaging MS apparatus named iMScope to identify the dominant molecules present in the human gastric mucosa near the fundic glands. Five gastric specimens were subjected to iMScope analysis. These specimens were also analyzed by immunohistochemistry using MUC5AC, H(+)-K(+)-ATPaseβ Claudin18 antibodies. RESULTS: Three major molecules with m/z 725.5, 780.5, and 782.5 detected in the gastric mucosa were identified as sphingomyelin (SM) (d18:1/16:0), phosphatidylcholine (PC) (16:0/18:2), and PC (16:0/18:1), respectively, through MS/MS analyses. Using immunohistological staining, SM (d18:1/16:0) signals were mainly co-localized with the foveolar epithelium marker MUC5AC. In contrast, PC (16:0/18:2) signals were observed in the region testing positive for the fundic gland marker H(+)-K(+)-ATPaseβ. PC (16:0/18:1) signals were uniformly distributed throughout the mucosa. CONCLUSION: Our basic data will contribute to the studies of lipid species in physical and pathological conditions of the human stomach.
AIM: To analyze the lipid distribution in gastric mucosae. METHODS: Imaging mass spectrometry (MS) is a useful tool to survey the distribution of biomolecules in surgical specimens. Here we used the imaging MS apparatus named iMScope to identify the dominant molecules present in the human gastric mucosa near the fundic glands. Five gastric specimens were subjected to iMScope analysis. These specimens were also analyzed by immunohistochemistry using MUC5AC, H(+)-K(+)-ATPaseβ Claudin18 antibodies. RESULTS: Three major molecules with m/z 725.5, 780.5, and 782.5 detected in the gastric mucosa were identified as sphingomyelin (SM) (d18:1/16:0), phosphatidylcholine (PC) (16:0/18:2), and PC (16:0/18:1), respectively, through MS/MS analyses. Using immunohistological staining, SM (d18:1/16:0) signals were mainly co-localized with the foveolar epithelium marker MUC5AC. In contrast, PC (16:0/18:2) signals were observed in the region testing positive for the fundic gland marker H(+)-K(+)-ATPaseβ. PC (16:0/18:1) signals were uniformly distributed throughout the mucosa. CONCLUSION: Our basic data will contribute to the studies of lipid species in physical and pathological conditions of the human stomach.
Entities:
Keywords:
Gastric mucosa; Imaging mass spectrometry; Phosphatidylcholine; Sphingomyelin; iMScope
Authors: W Bernhard; H P Haagsman; T Tschernig; C F Poets; A D Postle; M E van Eijk; H von der Hardt Journal: Am J Respir Cell Mol Biol Date: 1997-07 Impact factor: 6.914
Authors: C A Reis; L David; P A Nielsen; H Clausen; K Mirgorodskaya; P Roepstorff; M Sobrinho-Simões Journal: Int J Cancer Date: 1997-02-20 Impact factor: 7.396
Authors: Manu V Chakravarthy; Irfan J Lodhi; Li Yin; Raghu R V Malapaka; H Eric Xu; John Turk; Clay F Semenkovich Journal: Cell Date: 2009-07-30 Impact factor: 41.582
Authors: Giuseppe Corona; Renato Cannizzaro; Gianmaria Miolo; Laura Caggiari; Mariangela De Zorzi; Ombretta Repetto; Agostino Steffan; Valli De Re Journal: Int J Mol Sci Date: 2018-03-07 Impact factor: 5.923