| Literature DB >> 27190247 |
Yu-Sheng Chen1, Hong-Ru Li2, Wei Zhang2, Zhi-Dan Hua2, Xiao-Hong Lin2, Meng-Qing Lin2, Wen-Sen Huang2, Li-Ping Huang2, Xiao-Li Yu2, Neng-Luan Xu2, Ming Lin2, Bao-Song Xie2, Xiao-Na Shen3, Jian-Feng Xie3, Yi Wang4, Meng Huang3, Yan-An Wu5, Xin-Lan Hu5.
Abstract
We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.Entities:
Keywords: Acute respiratory tract infections; bead-based suspension array; high-throughput screening; pathogen detection; unexplained pneumonia
Mesh：
Substances：
Year: 2016 PMID： 27190247 PMCID： PMC4994910 DOI： 10.1177/1535370216647128
Source DB: PubMed Journal: Exp Biol Med (Maywood) ISSN： 1535-3699