| Literature DB >> 27190185 |
Cindy M Liu1, Jessica L Prodger2, Aaron A R Tobian3, David Serwadda4, Ronald M Galiwango4, Fred Nalugoda4, Nehemiah Kighoma4, Joshua Mwinike4, Margaret Anyokorit4, Lance B Price5, Maria J Wawer6, Godfrey Kigozi4, Ronald H Gray6.
Abstract
The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene-based quantitative polymerase chain reaction analysis and sequencing. PrePex users had higher absolute abundance of all bacteria than uncircumcised men (P = .001), largely due to increased numbers of the following anaerobes: Porphyromonas (5.2 × 10(7) 16S rRNA gene copies/swab in the PrePex group and 1.1 × 10(6) 16S rRNA gene copies/swab in uncircumcised men; P = .002), Peptoniphilus (1.0 × 10(7) and 1.8 × 10(6) 16S rRNA gene copies/swab, respectively; P < .05), Anaerococcus (1.0 × 10(7) and 1.1 × 10(6) 16S rRNA gene copies/swab, respectively; P < .001), and Campylobacter ureolyticus (1.7 × 10(5) and 1.6 × 10(7)16S rRNA gene copies/swab, respectively; P < .001). The PrePex-associated increase in anaerobes may account for unpleasant odor and a possible heightened risk of tetanus.Entities:
Keywords: PrePex device; Uganda; anaerobes; male circumcision; penile microbiota; tetanus
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Year: 2016 PMID： 27190185 PMCID： PMC4957438 DOI： 10.1093/infdis/jiw182
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226