| Literature DB >> 27188559 |
Elizabeth B Kauffman1, Mary A Franke2, Laura D Kramer2,3.
Abstract
West Nile virus is the most widespread mosquito-borne virus in the world, and the most common cause of encephalitis in the USA. Surveillance for this medially important mosquito-borne pathogen is an important part of public health practice. Here we present protocols for testing environmental samples such as mosquitoes, nonvertebrate mammals, and birds for this virus, including RT-PCR, virus isolation in cell culture, and antigenic assays, as well as serologic assays for antibody detection.Entities:
Keywords: ELISA; Flavivirus; PRNT; RT-PCR; Surveillance; West Nile
Mesh：
Year: 2016 PMID： 27188559 DOI： 10.1007/978-1-4939-3670-0_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745