Nunzia Di Maggio1, Elisa Martella2,3, Steve Meikle4, Marta Columbaro5, Enrico Lucarelli2, Matteo Santin4, Andrea Banfi1. 1. Cell & Gene Therapy, Department of Biomedicine, Basel University & Department of Surgery, Basel University Hospital, Basel, Switzerland. 2. Osteoarticular Regeneration Laboratory, Rizzoli Orthopedic Institute, Bologna, Italy. 3. Department of Biomedical & Neuromotor Sciences (DIBINEM), University of Bologna, Italy. 4. BrightSTAR, Brighton Centre for Regenerative Medicine, University of Brighton, UK. 5. Musculoskeletal Cell Biology Laboratory, Rizzoli Orthopedic Institute, Bologna, Italy.
Abstract
AIM: Rapid and efficient magnetization of human bone marrow stromal cells (BMSC) through functionalized magnetic nanoparticles (MNP). METHODS: MNP were functionalized with poly(epsilon-lysine) dendrons exposing carboxybetaine residue (CB-MNP) to enhance binding to the cellular glycocalix. BMSC were incubated with CB-MNP or non-functionalized PAA-MNP for 5-30 min in suspension. RESULTS: CB-MNP functionalization increased the magnetization efficiency by threefold. Remarkably, 66% of cells were magnetized after only 5 min and the maximum efficiency of >80% was reached by 15 min. BMSC viability, proliferation and differentiation were not impaired: actually, adipogenic and osteogenic differentiation were even improved. CONCLUSION: Carboxybetaine-dendron functionalization ensured rapid and efficient BMSC magnetization and allowed innovative suspension labeling, with a potential for bypassing adhesion culture of progenitors for regenerative medicine.
AIM: Rapid and efficient magnetization of human bone marrow stromal cells (BMSC) through functionalized magnetic nanoparticles (MNP). METHODS: MNP were functionalized with poly(epsilon-lysine) dendrons exposing carboxybetaine residue (CB-MNP) to enhance binding to the cellular glycocalix. BMSC were incubated with CB-MNP or non-functionalized PAA-MNP for 5-30 min in suspension. RESULTS: CB-MNP functionalization increased the magnetization efficiency by threefold. Remarkably, 66% of cells were magnetized after only 5 min and the maximum efficiency of >80% was reached by 15 min. BMSC viability, proliferation and differentiation were not impaired: actually, adipogenic and osteogenic differentiation were even improved. CONCLUSION:Carboxybetaine-dendron functionalization ensured rapid and efficient BMSC magnetization and allowed innovative suspension labeling, with a potential for bypassing adhesion culture of progenitors for regenerative medicine.
Authors: Richard Harrison; Hilda Anaid Lugo Leija; Stephanie Strohbuecker; James Crutchley; Sarah Marsh; Chris Denning; Alicia El Haj; Virginie Sottile Journal: Stem Cell Res Ther Date: 2018-09-26 Impact factor: 6.832