M Rijkers1, P F van der Meer2, I J Bontekoe2, B B Daal2, D de Korte2,3, F W G Leebeek4, J Voorberg5, A J G Jansen1,4. 1. Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands. 2. Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands. 3. Department of Blood Cell Research, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands. 4. Department of Hematology, Erasmus University Medical Centre, Rotterdam, The Netherlands. 5. Department of Plasma Proteins, Sanquin-AMC Landsteiner Laboratory, Amsterdam, The Netherlands. j.voorberg@sanquin.nl.
Abstract
BACKGROUND AND OBJECTIVES: In mice, loss of sialic acid resulting in shedding of glycoprotein (GP) Ibα and GPV has been linked to platelet survival. The aim of this study was to determine whether loss of sialic acid and the GPIb-IX-V complex contributes to development of the platelet storage lesion (PSL) in human platelet concentrates (PCs). MATERIALS AND METHODS: PCs (stored in plasma (with or without Mirasol treatment); PAS-C or PAS-E) were stored at room temperature. Flow cytometry was used to monitor membrane expression of the GPIb-IX-V complex, CD62P, surface glycans and PS exposure. The functionality of stored platelets was determined employing aggregometry and ristocetin-induced VWF binding. RESULTS: Storage time of PCs in blood banks is limited to 7 days. During this time period, a minor but gradually increasing subpopulation of GPIbα-negative platelets was observed. Also, ristocetin-induced VWF binding was impaired in a small population of platelets. Mean surface expression of GPIbα and GPV remained stable until day 9, whereas CD62P expression increased; also a rapid decrease in ADP-induced aggregation was observed for PAS-C, PAS-E and Mirasol-treated PCs. Upon prolonged storage (>9 days), a slow decline in surface expression of GPIbα and GPV was observed; no major changes were observed in surface sialylation with the exception of Mirasol-treated platelets. CONCLUSION: In a small population of stored platelets, changes in GPIbα occur from day 2 onwards. Loss of sialic acid and subsequent shedding of GPIbα and GPV is not an early event during the development of the PSL.
BACKGROUND AND OBJECTIVES: In mice, loss of sialic acid resulting in shedding of glycoprotein (GP) Ibα and GPV has been linked to platelet survival. The aim of this study was to determine whether loss of sialic acid and the GPIb-IX-V complex contributes to development of the platelet storage lesion (PSL) in human platelet concentrates (PCs). MATERIALS AND METHODS: PCs (stored in plasma (with or without Mirasol treatment); PAS-C or PAS-E) were stored at room temperature. Flow cytometry was used to monitor membrane expression of the GPIb-IX-V complex, CD62P, surface glycans and PS exposure. The functionality of stored platelets was determined employing aggregometry and ristocetin-induced VWF binding. RESULTS: Storage time of PCs in blood banks is limited to 7 days. During this time period, a minor but gradually increasing subpopulation of GPIbα-negative platelets was observed. Also, ristocetin-induced VWF binding was impaired in a small population of platelets. Mean surface expression of GPIbα and GPV remained stable until day 9, whereas CD62P expression increased; also a rapid decrease in ADP-induced aggregation was observed for PAS-C, PAS-E and Mirasol-treated PCs. Upon prolonged storage (>9 days), a slow decline in surface expression of GPIbα and GPV was observed; no major changes were observed in surface sialylation with the exception of Mirasol-treated platelets. CONCLUSION: In a small population of stored platelets, changes in GPIbα occur from day 2 onwards. Loss of sialic acid and subsequent shedding of GPIbα and GPV is not an early event during the development of the PSL.
Authors: Maaike Rijkers; Anno Saris; Sebastiaan Heidt; Arend Mulder; Leendert Porcelijn; Frans H J Claas; Ruben Bierings; Frank W G Leebeek; A J Gerard Jansen; Gestur Vidarsson; Jan Voorberg; Masja de Haas Journal: Haematologica Date: 2018-06-01 Impact factor: 9.941
Authors: Anno Saris; Ivan Peyron; Pieter F van der Meer; Tor B Stuge; Jaap Jan Zwaginga; S Marieke van Ham; Anja Ten Brinke Journal: Front Immunol Date: 2018-06-05 Impact factor: 7.561
Authors: Maaike Rijkers; David Schmidt; Nina Lu; Cynthia S M Kramer; Sebastiaan Heidt; Arend Mulder; Leendert Porcelijn; Frans H J Claas; Frank W G Leebeek; A J Gerard Jansen; Ilse Jongerius; Sacha S Zeerleder; Gestur Vidarsson; Jan Voorberg; Masja de Haas Journal: Haematologica Date: 2018-09-27 Impact factor: 9.941
Authors: Maurice Swinkels; Ferdows Atiq; Petra E Bürgisser; Johan A Slotman; Adriaan B Houtsmuller; Cilia de Heus; Judith Klumperman; Frank W G Leebeek; Jan Voorberg; Arend Jan Gerard Jansen; Ruben Bierings Journal: Res Pract Thromb Haemost Date: 2021-09-14
Authors: Maaike Rijkers; Bart L van den Eshof; Pieter F van der Meer; Floris P J van Alphen; Dirk de Korte; Frank W G Leebeek; Alexander B Meijer; Jan Voorberg; A J Gerard Jansen Journal: Sci Rep Date: 2017-09-08 Impact factor: 4.379
Authors: Maurice Swinkels; Maaike Rijkers; Jan Voorberg; Gestur Vidarsson; Frank W G Leebeek; A J Gerard Jansen Journal: Front Immunol Date: 2018-04-30 Impact factor: 7.561