Nitima Tatiya-Aphiradee1,2, Waranya Chatuphonprasert2,3, Kanokwan Jarukamjorn1,2. 1. a Faculty of Pharmaceutical Sciences , Khon Kaen University , Khon Kaen , Thailand. 2. b Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB) , Khon Kaen University , Khon Kaen , Thailand. 3. c Faculty of Medicine , Mahasarakham University , Mahasarakham , Thailand.
Abstract
CONTEXT: Garcinia mangostana Linn. (Guttiferae) (GM) pericarp has been shown to exhibit good in vitro antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA); however, there is currently no available information regarding its in vivo antibacterial activity. OBJECTIVE: To examine in vivo antibacterial activity of G. mangostana extract against MRSA. MATERIALS AND METHODS: GM pericarp was extracted by ethanol (GM-EtOH) and methanol (GM-MeOH). The crude extracts were examined for in vitro antibacterial activity against MRSA using broth microdilution assay. The in vivo antibacterial activity of 10% GM-EtOH against MRSA was determined in a tape stripping mouse model of superficial skin infection for 9 days by evaluating transepidermal water loss (TEWL) and performing colony counts from cultured swabs. RESULTS: GM-EtOH showed greater in vitro activity against MRSA than GM-MeOH in broth microdilution assay with minimum inhibitory concentration 17 versus 20 μg/mL and minimum bactericidal concentration 30 versus 35 μg/mL, respectively. The GM-EtOH (13.20 ± 0.49%) contained α-mangostin more than the GM-MeOH (9.83 ± 0.30%). In the tape stripping mouse model, 10% GM-EtOH reduced the number of MRSA colonies (0-1) recovered from infected wounds (>100 colonies) on the first day of treatment, restored TEWL to normal levels on the fourth day, and had completely healed the wounds by day 9. CONCLUSION: GM-EtOH showed promising in vivo antibacterial activity against MRSA in a superficial skin infection model in mice. It is of interest to develop a topical formulation of GM-EtOH to further study its potential as a novel antibacterial agent.
CONTEXT: Garcinia mangostana Linn. (Guttiferae) (GM) pericarp has been shown to exhibit good in vitro antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA); however, there is currently no available information regarding its in vivo antibacterial activity. OBJECTIVE: To examine in vivo antibacterial activity of G. mangostana extract against MRSA. MATERIALS AND METHODS:GM pericarp was extracted by ethanol (GM-EtOH) and methanol (GM-MeOH). The crude extracts were examined for in vitro antibacterial activity against MRSA using broth microdilution assay. The in vivo antibacterial activity of 10% GM-EtOH against MRSA was determined in a tape stripping mouse model of superficial skin infection for 9 days by evaluating transepidermal water loss (TEWL) and performing colony counts from cultured swabs. RESULTS:GM-EtOH showed greater in vitro activity against MRSA than GM-MeOH in broth microdilution assay with minimum inhibitory concentration 17 versus 20 μg/mL and minimum bactericidal concentration 30 versus 35 μg/mL, respectively. The GM-EtOH (13.20 ± 0.49%) contained α-mangostin more than the GM-MeOH (9.83 ± 0.30%). In the tape stripping mouse model, 10% GM-EtOH reduced the number of MRSA colonies (0-1) recovered from infected wounds (>100 colonies) on the first day of treatment, restored TEWL to normal levels on the fourth day, and had completely healed the wounds by day 9. CONCLUSION:GM-EtOH showed promising in vivo antibacterial activity against MRSA in a superficial skin infection model in mice. It is of interest to develop a topical formulation of GM-EtOH to further study its potential as a novel antibacterial agent.
Authors: Gina Porras; François Chassagne; James T Lyles; Lewis Marquez; Micah Dettweiler; Akram M Salam; Tharanga Samarakoon; Sarah Shabih; Darya Raschid Farrokhi; Cassandra L Quave Journal: Chem Rev Date: 2020-11-09 Impact factor: 60.622
Authors: Joseph P Hoffmann; Jessica K Friedman; Yihui Wang; James B McLachlan; Mimi C Sammarco; Lisa A Morici; Chad J Roy Journal: Front Microbiol Date: 2020-01-23 Impact factor: 5.640
Authors: Dwi Winarni; Fitria Nikmatul Husna; Muhammad Farraz Syadzha; Raden Joko Kuncoroningrat Susilo; Suhailah Hayaza; Arif Nur Muhammad Ansori; Mochammad Amin Alamsjah; Muhamad Nur Ghoyatul Amin; Pugar Arga Christina Wulandari; Pratiwi Pudjiastuti; Khalijah Awang Journal: Scientifica (Cairo) Date: 2022-02-09