| Literature DB >> 27179776 |
Sabin Bhandari1, Ingunn Bakke1, J Kumar2, Vidar Beisvag1, Arne K Sandvik3, Liv Thommesen1, Andrea Varro2, Kristin G Nørsett4.
Abstract
Connective tissue growth factor (CTGF) has been reported in gastric adenocarcinoma and in carcinoid tumors. The aim of this study was to explore a possible link between CTGF and gastrin in gastric epithelial cells and to study the role of CTGF in gastrin induced migration and invasion of AGS-GR cells. The effects of gastrin were studied using RT-qPCR, Western blot and assays for migration and invasion. We report an association between serum gastrin concentrations and CTGF abundancy in the gastric corpus mucosa of hypergastrinemic subjects and mice. We found a higher expression of CTGF in gastric mucosa tissue adjacent to tumor compared to normal control tissue. We showed that gastrin induced expression of CTGF in gastric epithelial AGS-GR cells via MEK, PKC and PKB/AKT pathways. CTGF inhibited gastrin induced migration and invasion of AGS-GR cells. We conclude that CTGF expression is stimulated by gastrin and involved in remodeling of the gastric epithelium.Entities:
Keywords: Connective tissue growth factor; Gastric epithelial cells; Gastrin; Invasion; Migration
Mesh：
Substances：
Year: 2016 PMID： 27179776 DOI： 10.1016/j.bbrc.2016.05.052
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575