Literature DB >> 27175190

Development of an indirect ELISA for bovine mastitis using Sip protein of Streptococcus agalactiae.

R E Bu1, J L Wang2, C DebRoy3, J H Wu1, L G W Xi1, Y Liu1, Z Q Shen4.   

Abstract

The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked immunosorbent assay (ELISA) was developed using the purified Sip protein as a coating antigen, which could identify S. agalactiae specific antibody in sera. The coating antigen at a concentration of 3.125 μg/ml, serum diluted to 1:160, and HRP-conjugated secondary antibody concentration at 1:4000 was found to be most effective in exhibiting positive result. The ELISA was found to be highly specific for S. agalactiae that may be used for the detection of the pathogen in mastitis cases, for epidemiological studies and for surveillance.

Entities:  

Keywords:  Bovine mastitis; ELISA; Streptococcusagalactiae

Year:  2015        PMID： 27175190      PMCID： PMC4782699     

Source DB:  PubMed          Journal:  Iran J Vet Res        ISSN： 2252-0589            Impact factor:   1.376


  10 in total

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Journal:  Can Vet J       Date:  1997-07       Impact factor: 1.008

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Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

3.  Foxes as a potential wildlife reservoir for mecA-positive Staphylococci.

Authors:  Marianne Carson; Anna L Meredith; Darren J Shaw; Efstathios S Giotis; David H Lloyd; Anette Loeffler
Journal:  Vector Borne Zoonotic Dis       Date:  2012-03-26       Impact factor: 2.133

4.  [Prevalence of anal-vaginal colonization of Streptococcus agalactiae in third trimester of pregnancy and susceptibility to macrolides and lincosamides, in pregnant women controlled at Clínica Alemana Temuco, Southern Chile].

Authors:  Fernando Abarzúa; Carolina Argomedo; Arturo Meissner; Tatiana Díaz; Paulina Garrido; Samantha Fariña; Carolina Chahin
Journal:  Rev Chilena Infectol       Date:  2014-06       Impact factor: 0.520

5.  Nosocomial spread of multidrug-resistant group B streptococci with reduced penicillin susceptibility belonging to clonal complex 1.

Authors:  Noriyuki Nagano; Yukiko Nagano; Masami Toyama; Kouji Kimura; Takashi Tamura; Keigo Shibayama; Yoshichika Arakawa
Journal:  J Antimicrob Chemother       Date:  2011-12-29       Impact factor: 5.790

6.  Real-time PCR targeting the sip gene for detection of group B Streptococcus colonization in pregnant women at delivery.

Authors:  Hakon Bergseng; Lars Bevanger; Marite Rygg; Kare Bergh
Journal:  J Med Microbiol       Date:  2007-02       Impact factor: 2.472

7.  Comparative genetic study of group B streptococcal strains of human and bovine origin.

Authors:  A Dmitriev; L Tkáciková; A Suvorov; M Kantíková; I Mikula; A Totolyan
Journal:  Folia Microbiol (Praha)       Date:  1999       Impact factor: 2.099

8.  The costs of production diseases in dairy herds in England.

Authors:  M A Kossaibati; R J Esslemont
Journal:  Vet J       Date:  1997-07       Impact factor: 2.688

9.  Multicenter study of the mechanisms of resistance and clonal relationships of Streptococcus agalactiae isolates resistant to macrolides, lincosamides, and ketolides in Spain.

Authors:  J J Gonzalez; A Andreu
Journal:  Antimicrob Agents Chemother       Date:  2005-06       Impact factor: 5.191

10.  Expression and functional analysis of porcine aminopeptidase N produced in prokaryotic expression system.

Authors:  Boqi Liu; Guangxing Li; Xiuwen Sui; Jiechao Yin; Heng Wang; Xiaofeng Ren
Journal:  J Biotechnol       Date:  2009-02-14       Impact factor: 3.307
  10 in total
  2 in total

Review 1.  Technological interventions and advances in the diagnosis of intramammary infections in animals with emphasis on bovine population-a review.

Authors:  Sandip Chakraborty; Kuldeep Dhama; Ruchi Tiwari; Mohd Iqbal Yatoo; Sandip Kumar Khurana; Rekha Khandia; Ashok Munjal; Palanivelu Munuswamy; M Asok Kumar; Mithilesh Singh; Rajendra Singh; Vivek Kumar Gupta; Wanpen Chaicumpa
Journal:  Vet Q       Date:  2019-12       Impact factor: 3.320

Review 2.  Immunogenic Proteins of Group B Streptococcus-Potential Antigens in Immunodiagnostic Assay for GBS Detection.

Authors:  Anna Dobrut; Monika Brzychczy-Włoch
Journal:  Pathogens       Date:  2021-12-31
  2 in total

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