Draft Genome Sequence of Lactobacillus sakei Strain FBL1, a Probiotic Bacterium Isolated from Mukeunji, a Long-Fermented Kimchi, in South Korea.

This report describes the 2,032,158-bp draft genome sequence of Lactobacillus sakei (L. sakei) strain FBL1, isolated from mukeunji purchased at the Gwangju World Kimchi Culture Festival in 2012, South Korea. The total draft genome size was 2,032,158 bp with a G+C content of 41.2%.

GENOME ANNOUNCEMENT

Lactobacillus sakei (L. sakei) is a facultative heterofermentative and Gram-positive anaerobic lactic acid bacterium (LAB). This bacterium, originally described in rice alcohol or sake, is commonly found in fresh meat and fish and is used as a starter culture (1). The complete genome sequence of L. sakei 23K (GenBank accession no. CR936503.1) was reported in 2005 (2). It consists of the circular chromosome containing 1,884,661 bp and 1,883 protein coding genes.

The genome sequence of L. sakei strain FBL1 with bile acid tolerance, isolated from mukeunji, a long-fermented kimchi (3), was analyzed in this study. L. sakei FBL1 was isolated from commercial mukeunji and showed 99% identity with the L. sakei strain DGH5 (KF469177.2) 16S rRNA gene. Comparative genomic analysis revealed 84% similarity with L. sakei 23K.

Genomic DNA was extracted with the G-spin genomic extraction kit (Intron Biotechnology Co., Republic of Korea) and quality was evaluated by the Agilent 2100 bioanalyzer with the high-sensitivity DNA kit. Draft genome sequencing was performed using an Ion Torrent Personal Genome Machine (PGM) system (Life Technologies, Germany) and a 318 semiconductor chip with 400-bp sequencing reads. A total of 4,048,495 reads, with an average read length of 320 bp, were generated. Reference-based assembly was carried out using SPAdes version 3.1.0, with L. sakei 23K as the reference genome. Assembly of the reads resulted in 44 contigs (≥500 bp, 541 to 287,097 bp, N50 length 116,042 bp). The total draft genome size was 2,005,160 bp with a G+C content of 41.0%. A total of 1,972 coding sequences, 76 RNA genes, and 296 subsystems were revealed by the RAST server (4, 5). The genome includes the production of a colicin V synthesis protein, adhesion and multidrug resistance efflux pumps, as well as those containing resistance genes, beta-lactam antibiotics, bile salt (choloylglycine hydrolase), and fluoroquinolones. Compared to the L. sakei 23-K genome, about 32 genes are missing. Importantly, phage proteins, choline binding protein, beta-galactosidase, phosphotransferase systems, and cobalt-zinc-cadmium resistance protein existed in L. sakei strain FBL1.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited in DDBJ/ENA/GenBank under the accession number LSFF00000000. The version described in this paper is the first version, LSFF01000000.