Yan Liu1, Weiqiang Chen2, Pengcheng Zhang1, Xiaodong Jin2, Xinguo Liu2, Ping Li2, Feifei Li1, Hongpeng Zhang3, Guozhang Zou4, Qiang Li5. 1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou, China; University of Chinese Academy of Sciences, Beijing, China. 2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou, China. 3. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China. 4. National Center for Nanoscience and Technology of China, Beijing, China; Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences, Beijing, China. 5. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou, China. Electronic address: liqiang@impcas.ac.cn.
Abstract
BACKGROUND AND PURPOSE: Cell cycle phase could affect the cellular uptake of nanoparticles. Based on the fact that ionizing radiation exposure can delay cell cycle progression including inducing G2/M phase arrest, we propose that ionizing radiation exposure is a cell cycle phase-dependent targeting approach for intracellular delivery of nano-agents in tumor cells. MATERIALS AND METHODS: We synthesized luminescent gold nanoclusters (AuNCs) using a one-pot green synthetic method. Subsequently, we used the as-prepared AuNCs as both "nano-agents" and fluorescent trafficking probes for our study using human cervical carcinoma HeLa cells. Estimating the cellular uptake of AuNCs and cell cycle analysis were performed following X-rays irradiation and cell synchronization. RESULTS: Our work showed that X-rays irradiation could delay the division of HeLa cells and thereby enhance the retention of AuNCs in HeLa cells, which is a reverse strategy compared with other studies on synergistic nano-radiotherapy. Our results demonstrated that the cell cycle synchronization influenced the cellular uptake processes of AuNCs, suggesting that dynamic cell cycle progression could affect the cellular uptake kinetics of AuNCs. CONCLUSION: We consider that the radiation-induced cell division delay might provide a possible mechanism underlying the enhanced effect for the cellular uptake of AuNCs in irradiated HeLa cells.
BACKGROUND AND PURPOSE: Cell cycle phase could affect the cellular uptake of nanoparticles. Based on the fact that ionizing radiation exposure can delay cell cycle progression including inducing G2/M phase arrest, we propose that ionizing radiation exposure is a cell cycle phase-dependent targeting approach for intracellular delivery of nano-agents in tumor cells. MATERIALS AND METHODS: We synthesized luminescent gold nanoclusters (AuNCs) using a one-pot green synthetic method. Subsequently, we used the as-prepared AuNCs as both "nano-agents" and fluorescent trafficking probes for our study using human cervical carcinoma HeLa cells. Estimating the cellular uptake of AuNCs and cell cycle analysis were performed following X-rays irradiation and cell synchronization. RESULTS: Our work showed that X-rays irradiation could delay the division of HeLa cells and thereby enhance the retention of AuNCs in HeLa cells, which is a reverse strategy compared with other studies on synergistic nano-radiotherapy. Our results demonstrated that the cell cycle synchronization influenced the cellular uptake processes of AuNCs, suggesting that dynamic cell cycle progression could affect the cellular uptake kinetics of AuNCs. CONCLUSION: We consider that the radiation-induced cell division delay might provide a possible mechanism underlying the enhanced effect for the cellular uptake of AuNCs in irradiated HeLa cells.
Authors: R C Popescu; D Savu; I Dorobantu; B S Vasile; H Hosser; A Boldeiu; M Temelie; M Straticiuc; D A Iancu; E Andronescu; F Wenz; F A Giordano; C Herskind; M R Veldwijk Journal: Sci Rep Date: 2020-06-29 Impact factor: 4.379
Authors: Roxana Cristina Popescu; Mihai Straticiuc; Cosmin Mustăciosu; Mihaela Temelie; Roxana Trușcă; Bogdan Ștefan Vasile; Adina Boldeiu; Dragoş Mirea; Radu Florin Andrei; Constantin Cenușă; Laurenţiu Mogoantă; George Dan Mogoșanu; Ecaterina Andronescu; Mihai Radu; Marlon R Veldwijk; Diana Iulia Savu Journal: Int J Mol Sci Date: 2020-09-30 Impact factor: 5.923