| Literature DB >> 27173027 |
Marialuisa Sponziello1, Francesca Rosignolo1, Marilena Celano2, Valentina Maggisano2, Valeria Pecce1, Roberta Francesca De Rose2, Giovanni Enrico Lombardo2, Cosimo Durante1, Sebastiano Filetti1, Giuseppe Damante3, Diego Russo4, Stefania Bulotta2.
Abstract
In this study we analyzed the expression levels of markers of epithelial-to-mesenchymal transition (EMT) in several papillary thyroid carcinomas (PTCs) and the relation with tumor genotypes and clinicopathological characteristics. The role of fibronectin-1 (FN1) was investigated by analyzing the effects of FN1 silencing in two human thyroid cancer cell lines. Most of EMT markers were significantly over-expressed in a group of 36 PTCs. In particular, FN1 mRNA levels were higher in tumor vs non-tumor tissue (117.3, p < 0.001) and also in aggressive and BRAF(V600E) samples. Similar results were observed (and confirmed at the protein level) when FN1 expression was analyzed in a validation group of 50 PTCs and six lymph node (LN) metastases. Silencing of FN1 in TPC-1 and BCPAP thyroid cancer cells significantly reduced proliferation, adhesion, migration, and invasion in both cell lines. Collectively, our data indicate that FN1 overexpression is an important determinant of thyroid cancer aggressiveness.Entities:
Keywords: BRAF; EMT; Fibronectin; Thyroid cancer
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Year: 2016 PMID： 27173027 DOI： 10.1016/j.mce.2016.05.007
Source DB: PubMed Journal: Mol Cell Endocrinol ISSN： 0303-7207 Impact factor: 4.102