Asker Zeki Özsoy1, Ayşe Feyda Nursal2, Akgül Arıcı3, İlknur Bütün4, Murat Uysal5, Hilal Irmak Sapmaz5, Çiğdem Kunt İşgüder6, Hatice Yılmaz Doğru6, Ufuk Taş5. 1. Faculty of Medicine, Department of Obstetrics and Gynecology, Gaziosmanpaşa University, Tokat, Turkey. drzekiozsoy@hotmail.com. 2. Faculty of Medicine, Department of Medical Genetic, Giresun University, Giresun, Turkey. 3. Faculty of Medicine, Department of Medical Pathology, Gaziosmanpaşa University, Tokat, Turkey. 4. Faculty of Medicine, Department of Medical Biochemistry, Gaziosmanpaşa University, Tokat, Turkey. 5. Faculty of Medicine, Department of Anatomy, Gaziosmanpaşa University, Tokat, Turkey. 6. Faculty of Medicine, Department of Obstetrics and Gynecology, Gaziosmanpaşa University, Tokat, Turkey.
Abstract
AIM: The aim of this study was to investigate the effects of carvedilol (CVD) on experimentally induced ovarian ischemia/reperfusion (I/R) injury in rats. METHODS: An ovarian I/R model was applied to rats, classified into three groups: 1 (n = 7), sham operated (control); 2 (n = 7), 3 h ischemia + 3 h reperfusion (I/R); 3 (n = 7), 3 h ischemia + CVD + 3 h reperfusion (I/R + CVD). Malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities in ovarian tissues and serum were measured. Tissue damage was examined histopathologically; Bax and caspase-3 expression was determined immunhistochemically. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed to show apoptotic cell death. RESULTS: MDA levels in ovarian tissues were significantly increased in the I/R group compared with the control. CVD administration significantly decreased tissue MDA levels in the I/R + CVD in comparison with the I/R group. GSH-Px activities in serum were higher in the I/R + CVD than in the I/R group. SOD activities in tissue and serum were significantly decreased in the I/R compared with the control group. Histological examination showed a significant improvement in ovarian morphology in the I/R + CVD compared with the I/R group. Bax and caspase-3 protein was more strongly expressed in the I/R group compared with the control and I/R + CVD groups. Apoptotic index detected by TUNEL assay was significantly increased in the I/R and decreased in the I/R + CVD group. CONCLUSION: Our results suggest that CVD reduces the deleterious effects of oxidative damage on ovaries in a rat I/R model.
AIM: The aim of this study was to investigate the effects of carvedilol (CVD) on experimentally induced ovarian ischemia/reperfusion (I/R) injury in rats. METHODS: An ovarian I/R model was applied to rats, classified into three groups: 1 (n = 7), sham operated (control); 2 (n = 7), 3 h ischemia + 3 h reperfusion (I/R); 3 (n = 7), 3 h ischemia + CVD + 3 h reperfusion (I/R + CVD). Malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities in ovarian tissues and serum were measured. Tissue damage was examined histopathologically; Bax and caspase-3 expression was determined immunhistochemically. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed to show apoptotic cell death. RESULTS: MDA levels in ovarian tissues were significantly increased in the I/R group compared with the control. CVD administration significantly decreased tissue MDA levels in the I/R + CVD in comparison with the I/R group. GSH-Px activities in serum were higher in the I/R + CVD than in the I/R group. SOD activities in tissue and serum were significantly decreased in the I/R compared with the control group. Histological examination showed a significant improvement in ovarian morphology in the I/R + CVD compared with the I/R group. Bax and caspase-3 protein was more strongly expressed in the I/R group compared with the control and I/R + CVD groups. Apoptotic index detected by TUNEL assay was significantly increased in the I/R and decreased in the I/R + CVD group. CONCLUSION: Our results suggest that CVD reduces the deleterious effects of oxidative damage on ovaries in a rat I/R model.
Authors: Caren Nádia Soares de Sousa; Ingridy da Silva Medeiros; Germana Silva Vasconcelos; Gabriel Angelo de Aquino; Francisco Maurício Sales Cysne Filho; Jamily Cunha de Almeida Cysne; Danielle Silveira Macêdo; Silvânia Maria Mendes Vasconcelos Journal: Psychopharmacology (Berl) Date: 2022-01-13 Impact factor: 4.530