Hideki Osawa1, Junichi Nishimura2, Masayuki Hiraki1, Hidekazu Takahashi1, Naotsugu Haraguchi1, Taishi Hata1, Masakazu Ikenaga1,3, Kohei Murata1,4, Hirofumi Yamamoto1, Tsunekazu Mizushima1, Yuichiro Doki1, Masaki Mori1. 1. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, E2-2-2 Yamada-oka, Suita, 565-0871, Osaka, Japan. 2. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, E2-2-2 Yamada-oka, Suita, 565-0871, Osaka, Japan. jnishimura@gesurg.med.osaka-u.ac.jp. 3. Department of Surgery, Higashiosaka City General Hospital, 3-4-5, Nishiiwata, Higashiosaka, 578-8588, Osaka, Japan. 4. Department of Surgery, Suita Municipal Hospital, 2-13-20 Katayama-cho, Suita, 564-0082, Osaka, Japan.
Abstract
PURPOSE: To examine the regeneration of mesothelium under a bioresorbable membrane. METHODS: A 1 cm2 piece of peritoneum was resected from both sides of the abdominal wall of retired female mice. A piece of hyaluronate and carboxymethyl-cellulose (Seprafilm®) was placed over the wound on one side and the other side was left uncovered. We evaluated the degree of adhesion and regeneration of mesothelial cells macroscopically and histologically using immunohistochemistry at different times. RESULTS: Macroscopically, the degree of postoperative adhesion in the treated site was significantly less than that in the untreated site. The membrane was left in place for 7 postoperative days (PODs). By POD 5, the regenerated peritoneum mesothelial cells covered part of the area and by POD 7, they had regenerated over almost all of that area in the abdominal wall. CONCLUSION: The anti-adhesion membrane worked as a physical barrier to prevent postoperative adhesion until the mesothelial cells had regenerated completely. To our knowledge, this is the first study conducted to assess the regeneration of peritoneum mesothelial cells under a bioresorbable membrane using immunohistochemistry.
PURPOSE: To examine the regeneration of mesothelium under a bioresorbable membrane. METHODS: A 1 cm2 piece of peritoneum was resected from both sides of the abdominal wall of retired female mice. A piece of hyaluronate and carboxymethyl-cellulose (Seprafilm®) was placed over the wound on one side and the other side was left uncovered. We evaluated the degree of adhesion and regeneration of mesothelial cells macroscopically and histologically using immunohistochemistry at different times. RESULTS: Macroscopically, the degree of postoperative adhesion in the treated site was significantly less than that in the untreated site. The membrane was left in place for 7 postoperative days (PODs). By POD 5, the regenerated peritoneum mesothelial cells covered part of the area and by POD 7, they had regenerated over almost all of that area in the abdominal wall. CONCLUSION: The anti-adhesion membrane worked as a physical barrier to prevent postoperative adhesion until the mesothelial cells had regenerated completely. To our knowledge, this is the first study conducted to assess the regeneration of peritoneum mesothelial cells under a bioresorbable membrane using immunohistochemistry.
Authors: Terry T W Leung; Elijah Dixon; Manpreet Gill; Brett D Mador; Kyle M Moulton; Gilaad G Kaplan; Anthony R MacLean Journal: Ann Surg Date: 2009-07 Impact factor: 12.969