Qi Fu1,2,3, Yong Du1,2, Chun Yang2, Dong Zhang2, Ningmei Zhang4, Xiaoming Liu1, William C Cho5, Yinxue Yang1,2. 1. a Human Stem Cell Institute , General Hospital of Ningxia Medical University , Yinchuan , China. 2. b Department of Colorectal Surgery , General Hospital of Ningxia Medical University , Yinchuan , China. 3. c Graduate School , Ningxia Medical University , Yinchuan , China. 4. d Department of Pathology , General Hospital of Ningxia Medical University , Yinchuan , China. 5. e Department of Clinical Oncology , Queen Elizabeth Hospital , Kowloon , Hong Kong.
Abstract
OBJECTIVE: A microRNA (miRNA) that functionally downregulates the expression of tumor suppressors can be defined as an oncomir. Here, we interrogate the biological significance of miR-592 in colorectal cancer (CRC). RESEARCH DESIGN AND METHODS: The expression of miR-592 in CRC tissues and cell lines was ascertained by qRT-PCR assay, and the expression of its target gene was determined by immunohistochemistry staining. The oncogenic role of miR-592 was assessed in terms of cell proliferation, migration, and clonogenicity in vitro, whereas the tumorigenicity was assessed by inhibiting endogenous miR-592 in CRC cells in vivo. RESULTS: A striking upregulation of miR-592 was observed in CRC tissues and cell lines compared to the matched adjacent non-tumor tissues and normal colon cells. Importantly, Forkhead Box O3A (FoxO3A) was identified as a novel target of miR-592. miR-592 inhibitor exhibited a significant reduction of migration, proliferation, and clonogenicity in CRC cells. These cells also displayed a decreased tumorigenicity in SCID mice relative to the control cells. CONCLUSION: These data suggest that miR-592 may promote the progression and metastasis, in part, by targeting FoxO3A in CRC. miR-592 may be a novel target for CRC treatment and antagomir-592 may inhibit the proliferation and metastasis of CRC cells.
OBJECTIVE: A microRNA (miRNA) that functionally downregulates the expression of tumor suppressors can be defined as an oncomir. Here, we interrogate the biological significance of miR-592 in colorectal cancer (CRC). RESEARCH DESIGN AND METHODS: The expression of miR-592 in CRC tissues and cell lines was ascertained by qRT-PCR assay, and the expression of its target gene was determined by immunohistochemistry staining. The oncogenic role of miR-592 was assessed in terms of cell proliferation, migration, and clonogenicity in vitro, whereas the tumorigenicity was assessed by inhibiting endogenous miR-592 in CRC cells in vivo. RESULTS: A striking upregulation of miR-592 was observed in CRC tissues and cell lines compared to the matched adjacent non-tumor tissues and normal colon cells. Importantly, Forkhead Box O3A (FoxO3A) was identified as a novel target of miR-592. miR-592 inhibitor exhibited a significant reduction of migration, proliferation, and clonogenicity in CRC cells. These cells also displayed a decreased tumorigenicity in SCIDmice relative to the control cells. CONCLUSION: These data suggest that miR-592 may promote the progression and metastasis, in part, by targeting FoxO3A in CRC. miR-592 may be a novel target for CRC treatment and antagomir-592 may inhibit the proliferation and metastasis of CRC cells.
Authors: Ronja S Adam; Dennis Poel; Leandro Ferreira Moreno; Joey M A Spronck; Tim R de Back; Arezo Torang; Patricia M Gomez Barila; Sanne Ten Hoorn; Florian Markowetz; Xin Wang; Henk M W Verheul; Tineke E Buffart; Louis Vermeulen Journal: Mol Oncol Date: 2022-04-29 Impact factor: 7.449
Authors: A Setién-Olarra; X Marichalar-Mendia; N G Bediaga; P Aguirre-Echebarria; J M Aguirre-Urizar; A Mosqueda-Taylor Journal: PLoS One Date: 2017-10-20 Impact factor: 3.240