Ufuk Ates1, Gulnur Gollu2, Gonul Kucuk2, Deniz Billur3, Meltem Bingol-Kologlu2, Yavuz Yılmaz4, Hulya Ozkan-Ulu4, Pinar Bayram3, Emin Bagriacik5, Huseyin Dindar2. 1. Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey. drufukates@gmail.com. 2. Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey. 3. Ankara University School of Medicine, Department of Histology and Embryology, Ankara, Turkey. 4. Zekai Tahir Burak Woman Health Education and Research Hospital, Department of Pediatric Surgery, Ankara, Turkey. 5. Gazi University, School of Medicine, Immunology Research Center, Ankara, Turkey.
Abstract
BACKGROUND/AIM: The aim of the present study was to find out if there is an increase in the expression of pro-apoptotic Bax and reduction in expression of anti-apoptotic Blc-2A1 in newborn intestines with necrotizing enterocolitis (NEC). MATERIALS AND METHODS: We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry. RESULTS: We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry. CONCLUSIONS: Our data in humannewborns suggest that alteration of the balance between pro-apoptotic Bax expression and anti-apoptotic Bcl-2A1 expression in the site of injury is a possible mechanism in the pathogenesis of NEC. Sociedad Argentina de Pediatría.
BACKGROUND/AIM: The aim of the present study was to find out if there is an increase in the expression of pro-apoptotic Bax and reduction in expression of anti-apoptotic Blc-2A1 in newborn intestines with necrotizing enterocolitis (NEC). MATERIALS AND METHODS: We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry. RESULTS: We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry. CONCLUSIONS: Our data in humannewborns suggest that alteration of the balance between pro-apoptotic Bax expression and anti-apoptotic Bcl-2A1 expression in the site of injury is a possible mechanism in the pathogenesis of NEC. Sociedad Argentina de Pediatría.
Authors: Ilse H de Lange; Charlotte van Gorp; Laurens D Eeftinck Schattenkerk; Wim G van Gemert; Joep P M Derikx; Tim G A M Wolfs Journal: Nutrients Date: 2021-05-19 Impact factor: 5.717
Authors: Adam D Werts; William B Fulton; Mitchell R Ladd; Ali Saad-Eldin; Yue X Chen; Mark L Kovler; Hongpeng Jia; Emilyn C Banfield; Rachael H Buck; Karen Goehring; Thomas Prindle; Sanxia Wang; Qinjie Zhou; Peng Lu; Yukihiro Yamaguchi; Chhinder P Sodhi; David J Hackam Journal: Cell Mol Gastroenterol Hepatol Date: 2019-11-19