| Literature DB >> 27163731 |
Joon Ki Kim1, Kyoung Ah Kang2, Mei Jing Piao2, Yea Seong Ryu2, Xia Han2, Pattage Madushan Dilhara Jayatissa Fernando2, Min Chang Oh2, Jeong Eon Park2, Kristina Shilnikova2, Sun Jin Boo3, Soo-Young Na3, Yong Joo Jeong1, Seung Uk Jeong4, Jin Won Hyun5.
Abstract
Colon cancer can be treated with 5-fluorouracil (5-FU), but 5-FU resistance frequently occurs. We determined whether 5-FU resistance arises as a result of endoplasmic reticulum (ER) stress. 5-FU-resistant SNUC5 colon cancer cells (SNUC5/FUR cells) expressed higher levels of ER stress-related proteins than drug-sensitive SNUC5 cells. SNUC5/FUR cells also exhibited more intense ER staining and higher level of mitochondrial Ca(2+) overload. SNUC5/FUR cells transfected with siRNA against GRP78, ATF6, ERK, or AKT were more sensitive to 5-FU than siControl RNA-transfected cells. These results suggested that 5-FU resistance was associated with ER stress in colon cancer.Entities:
Keywords: 5-FU resistance; ER stress; ER stress-related proteins; Extracellular signal-regulated kinases; Human colon cancer cells
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Year: 2016 PMID： 27163731 DOI： 10.1016/j.etap.2016.05.005
Source DB: PubMed Journal: Environ Toxicol Pharmacol ISSN： 1382-6689 Impact factor: 4.860