Ruowen Zhang1, Yilin Wang2, Dong Zhu3, Fengying Dong4, Xi Chen5. 1. Beihua University Faculty of Medicine, Beihua University, Jilin, China. 2. The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. 3. The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. 4. Jilin Gynecology and Obstetrics Hospital, Jilin, China. 5. Pathogen Biology Department, College of Basic Medicine, Beihua University, Jilin, China.
Abstract
BACKGROUND: In this study, the authors cultivated ECV-304 in vitro and incubated cells with H2O2, established injury models, and induced oxidized endothelial cell apoptosis. This model makes it possible to choose suitable concentrations of North Schisandra Lignans. OBJECTIVE: To study the protective effects of North Schisandra Lignans on human umbilical vein endothelial cell injuries. METHODS: Endothelial cell growth and proliferation activity were detected through the MTT method. The colorimetric method was used to determine superoxide dismutase (SOD) activity in the cell culture solution, as well as malondialdehyde (MDA) content in the cell. RESULTS: North Schisandra Lignans noticeably decreased ECV-304 cell injury induced by H2O2. Moderate and high concentrations of North Schisandra Lignans could significantly lower MDA content and heighten SOD activity. These differences were significant compared to the H2O2 group (P< 0.05). CONCLUSIONS: North Schisandra Lignans had an obvious protective effect on ECV-304 injured by H2O2$. The mechanism decreases MDA production and heightened SOD activity.
BACKGROUND: In this study, the authors cultivated ECV-304 in vitro and incubated cells with H2O2, established injury models, and induced oxidized endothelial cell apoptosis. This model makes it possible to choose suitable concentrations of North Schisandra Lignans. OBJECTIVE: To study the protective effects of North Schisandra Lignans on human umbilical vein endothelial cell injuries. METHODS: Endothelial cell growth and proliferation activity were detected through the MTT method. The colorimetric method was used to determine superoxide dismutase (SOD) activity in the cell culture solution, as well as malondialdehyde (MDA) content in the cell. RESULTS: North Schisandra Lignans noticeably decreased ECV-304 cell injury induced by H2O2. Moderate and high concentrations of North Schisandra Lignans could significantly lower MDA content and heighten SOD activity. These differences were significant compared to the H2O2 group (P< 0.05). CONCLUSIONS: North Schisandra Lignans had an obvious protective effect on ECV-304 injured by H2O2$. The mechanism decreases MDA production and heightened SOD activity.
Entities:
Keywords:
North Schisandra Lignans; malondialdehyde; superoxide dismutase; vascular endothelial cell