Tomoya Kawaguchi1, Yasuhiro Koh2, Masahiko Ando2, Norimasa Ito2, Sadanori Takeo2, Hirofumi Adachi2, Tsutomu Tagawa2, Seiichi Kakegawa2, Motohiro Yamashita2, Kazuhiko Kataoka2, Yukito Ichinose2, Yukiyasu Takeuchi2, Masakuni Serizawa2, Akihiro Tamiya2, Shigeki Shimizu2, Naoki Yoshimoto2, Akihito Kubo2, Shun-Ichi Isa2, Hideo Saka2, Akihide Matsumura2. 1. Tomoya Kawaguchi, Akihiro Tamiya, Shigeki Shimizu, Shun-ichi Isa, and Akihide Matsumura, National Hospital Organization Kinki-chuo Chest Medical Center; Tomoya Kawaguchi and Naoki Yoshimoto, Osaka City University, Osaka; Yasuhiro Koh, Seiichi Kakegawa, Masakuni Serizawa, Akihito Kubo, and Hideo Saka, National Hospital Organization Nagoya Medical Center; Masahiko Ando, Nagoya University, Nagoya; Yasuhiro Koh, Wakayama Medical University, Wakayama; Yasuhiro Koh and Masakuni Serizawa, Shizuoka Cancer Center Research Institute, Shizuoka; Norimasa Ito, National Hospital Organization Matsue Medical Center, Matsue; Sadanori Takeo, National Hospital Organization Kyushu Medical Center; Yukito Ichinose, National Kyushu Cancer Center, Fukuoka; Hirofumi Adachi, National Hospital Organization Hokkaido Cancer Center, Hokkaido; Tsutomu Tagawa, National Hospital Organization Nagasaki Medical Center, Omura; Seiichi Kakegawa, National Hospital Organization Nishigunma National Hospital, Shibukawa; Motohiro Yamashita, National Hospital Organization Shikoku Cancer Center, Matsuyama; Kazuhiko Kataoka, National Hospital Organization Iwakuni Clinical Center, Iwakuni; Yukiyasu Takeuchi, National Hospital Organization Toneyama National Hospital, Toyonaka; Shigeki Shimizu, Hyogo Medical Collage, Hyogo; and Akihito Kubo, Aichi Medical University School of Medicine, Aichi, Japan. kawaguchi.tomoya@med.osaka-cu.ac.jp. 2. Tomoya Kawaguchi, Akihiro Tamiya, Shigeki Shimizu, Shun-ichi Isa, and Akihide Matsumura, National Hospital Organization Kinki-chuo Chest Medical Center; Tomoya Kawaguchi and Naoki Yoshimoto, Osaka City University, Osaka; Yasuhiro Koh, Seiichi Kakegawa, Masakuni Serizawa, Akihito Kubo, and Hideo Saka, National Hospital Organization Nagoya Medical Center; Masahiko Ando, Nagoya University, Nagoya; Yasuhiro Koh, Wakayama Medical University, Wakayama; Yasuhiro Koh and Masakuni Serizawa, Shizuoka Cancer Center Research Institute, Shizuoka; Norimasa Ito, National Hospital Organization Matsue Medical Center, Matsue; Sadanori Takeo, National Hospital Organization Kyushu Medical Center; Yukito Ichinose, National Kyushu Cancer Center, Fukuoka; Hirofumi Adachi, National Hospital Organization Hokkaido Cancer Center, Hokkaido; Tsutomu Tagawa, National Hospital Organization Nagasaki Medical Center, Omura; Seiichi Kakegawa, National Hospital Organization Nishigunma National Hospital, Shibukawa; Motohiro Yamashita, National Hospital Organization Shikoku Cancer Center, Matsuyama; Kazuhiko Kataoka, National Hospital Organization Iwakuni Clinical Center, Iwakuni; Yukiyasu Takeuchi, National Hospital Organization Toneyama National Hospital, Toyonaka; Shigeki Shimizu, Hyogo Medical Collage, Hyogo; and Akihito Kubo, Aichi Medical University School of Medicine, Aichi, Japan.
Abstract
PURPOSE: Oncogenic driver mutations are critical for lung cancer development and serve as therapeutic targets. However, their associations with environmental factors are not fully understood. We aimed to elucidate the relationship between tumor developmental biology and exposure to environmental factors. PATIENTS AND METHODS: This was a prospective, multicenter, molecular epidemiology study. Eligible patients were those with newly diagnosed stages I to IIIB non-small-cell lung cancer (NSCLC) who underwent surgery. The tumors were examined for somatic mutations in 72 cancer-associated genes by targeted deep sequencing, estrogen receptor β (ERβ) expression using immunohistochemical staining, and infection with any of 37 types of human papillomavirus (HPV) using a polymerase chain reaction-based microarray system. Detailed information on patient demographics and environmental factors was obtained from a comprehensive questionnaire. RESULTS: From July 2012 to December 2013, 957 patients were enrolled, and molecular analyses were performed on 876 samples (from 441 ever- and 435 never-smokers). Oncogenic driver mutations in P53 and KRAS increased proportionally with smoking status, whereas mutations in EGFR and SMAD4 decreased. KRAS mutations in smokers and SMAD4 mutations were observed more frequently in proportion to body mass index. TP53 and NFE2L2 mutations were observed more frequently in advanced NSCLC stages. As for never-smokers, no environmental factors were significantly associated with mutational changes. EGFR mutations and TP53 mutations were observed more frequently in women and in men, respectively. Mutations in these two genes were also potentially associated with ERβ expression. Only three patients (0.3%) were HPV positive. CONCLUSION: The mutational spectrum is associated with smoking, body mass index, and other environmental factors, as well as with ERβ expression. Little association was observed between HPV and NSCLC.
PURPOSE: Oncogenic driver mutations are critical for lung cancer development and serve as therapeutic targets. However, their associations with environmental factors are not fully understood. We aimed to elucidate the relationship between tumor developmental biology and exposure to environmental factors. PATIENTS AND METHODS: This was a prospective, multicenter, molecular epidemiology study. Eligible patients were those with newly diagnosed stages I to IIIB non-small-cell lung cancer (NSCLC) who underwent surgery. The tumors were examined for somatic mutations in 72 cancer-associated genes by targeted deep sequencing, estrogen receptor β (ERβ) expression using immunohistochemical staining, and infection with any of 37 types of human papillomavirus (HPV) using a polymerase chain reaction-based microarray system. Detailed information on patient demographics and environmental factors was obtained from a comprehensive questionnaire. RESULTS: From July 2012 to December 2013, 957 patients were enrolled, and molecular analyses were performed on 876 samples (from 441 ever- and 435 never-smokers). Oncogenic driver mutations in P53 and KRAS increased proportionally with smoking status, whereas mutations in EGFR and SMAD4 decreased. KRAS mutations in smokers and SMAD4 mutations were observed more frequently in proportion to body mass index. TP53 and NFE2L2 mutations were observed more frequently in advanced NSCLC stages. As for never-smokers, no environmental factors were significantly associated with mutational changes. EGFR mutations and TP53 mutations were observed more frequently in women and in men, respectively. Mutations in these two genes were also potentially associated with ERβ expression. Only three patients (0.3%) were HPV positive. CONCLUSION: The mutational spectrum is associated with smoking, body mass index, and other environmental factors, as well as with ERβ expression. Little association was observed between HPV and NSCLC.
Authors: Shigeki Nanjo; Wei Wu; Niki Karachaliou; Collin M Blakely; Junji Suzuki; Yu-Ting Chou; Siraj M Ali; D Lucas Kerr; Victor R Olivas; Jonathan Shue; Julia Rotow; Manasi K Mayekar; Franziska Haderk; Nilanjana Chatterjee; Anatoly Urisman; Jia Chi Yeo; Anders J Skanderup; Aaron C Tan; Wai Leong Tam; Oscar Arrieta; Kazuyoshi Hosomichi; Akihiro Nishiyama; Seiji Yano; Yuriy Kirichok; Daniel Sw Tan; Rafael Rosell; Ross A Okimoto; Trever G Bivona Journal: J Clin Invest Date: 2022-07-01 Impact factor: 19.456
Authors: Caroline A Thompson; Scarlett Lin Gomez; Katherine G Hastings; Kristopher Kapphahn; Peter Yu; Salma Shariff-Marco; Ami S Bhatt; Heather A Wakelee; Manali I Patel; Mark R Cullen; Latha P Palaniappan Journal: Cancer Epidemiol Biomarkers Prev Date: 2016-10 Impact factor: 4.254
Authors: Ting-Yuan David Cheng; Amy K Darke; Mary W Redman; Gary R Zirpoli; Warren Davis; Rochelle Payne Ondracek; Wiam Bshara; Angela R Omilian; Robert Kratzke; Mary E Reid; Julian R Molina; Jill M Kolesar; Yuhchyau Chen; Robert M MacRae; James Moon; Philip Mack; David R Gandara; Karen Kelly; Regina M Santella; Kathy S Albain; Christine B Ambrosone Journal: J Natl Cancer Inst Date: 2018-07-01 Impact factor: 13.506
Authors: Edward B Garon; Jill M Siegfried; Laura P Stabile; Patricia A Young; Diana C Marquez-Garban; David J Park; Ravi Patel; Eddie H Hu; Saeed Sadeghi; Rupesh J Parikh; Karen L Reckamp; Brad Adams; Robert M Elashoff; David Elashoff; Tristan Grogan; He-Jing Wang; Sanja Dacic; Meghan Brennan; Yacgley Valdes; Simon Davenport; Steven M Dubinett; Michael F Press; Dennis J Slamon; Richard J Pietras Journal: Lung Cancer Date: 2018-06-22 Impact factor: 6.081