Yao Xin1, Jiang Wei2, Ma Chunhua3, Yu Danhong4, Zhu Jianguo1, Cheng Zongqi5, Bao Jian-An6. 1. Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, PR China. 2. Taizhou Institute for Food and Drug Control, Taizhou 225300, PR China. 3. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, PR China. 4. Soochow University Affiliated Children's Hospital, Suzhou 215003, PR China. 5. Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, PR China. Electronic address: Chengzqsuzhou@163.com. 6. Department of Pharmacy, The First Affiliated Hospital of Soochow University, Suzhou 215006, PR China. Electronic address: Baojasuzhou@126.com.
Abstract
BACKGROUND: AMP-activated protein kinase (AMPK) is one of the principal cellular energy sensors participating in maintenance of energy balance but recent evidences also suggested that AMPK might be involved in the regulation of inflammation. STUDY DESIGN/ METHODS: Ginsenoside Rg1 (Rg1) was used to investigate the potential roles of AMPK in carbon tetrachloride (CCl4)-induced hepato-toxicity. The experimental data indicated that treatment with Rg1 significantly decreased the elevation of plasma aminotransferases and alleviated hepatic histological abnormalities in CCl4-exposed mice. Treatment with Rg1 also inhibited the increase of myeloperoxidase (MPO) and malondialdehyde (MDA), the induction of TNF-α, IL-6, inducible nitric oxide synthase (iNOS), nitric oxide and the upregulation of matrix metalloproteinase 2 (MMP-2), MMP-3 and MMP-9 in mice exposed to CCl4. These effects were associated with suppressed nuclear accumulation of NF-κB p65. CONCLUSION: These results indicated that Rg1 effectively suppressed the inflammatory responses and alleviated liver damage induced by CCl4, implying that AMPK activation might be beneficial for ameliorating inflammation-based liver damage.
BACKGROUND: AMP-activated protein kinase (AMPK) is one of the principal cellular energy sensors participating in maintenance of energy balance but recent evidences also suggested that AMPK might be involved in the regulation of inflammation. STUDY DESIGN/ METHODS:GinsenosideRg1 (Rg1) was used to investigate the potential roles of AMPK in carbon tetrachloride (CCl4)-induced hepato-toxicity. The experimental data indicated that treatment with Rg1 significantly decreased the elevation of plasma aminotransferases and alleviated hepatic histological abnormalities in CCl4-exposed mice. Treatment with Rg1 also inhibited the increase of myeloperoxidase (MPO) and malondialdehyde (MDA), the induction of TNF-α, IL-6, inducible nitric oxide synthase (iNOS), nitric oxide and the upregulation of matrix metalloproteinase 2 (MMP-2), MMP-3 and MMP-9 in mice exposed to CCl4. These effects were associated with suppressed nuclear accumulation of NF-κB p65. CONCLUSION: These results indicated that Rg1 effectively suppressed the inflammatory responses and alleviated liver damage induced by CCl4, implying that AMPK activation might be beneficial for ameliorating inflammation-based liver damage.
Authors: Sang Mi Park; Eun Hye Jung; Jae Kwang Kim; Kyung Hwan Jegal; Chung A Park; Il Je Cho; Sang Chan Kim Journal: J Ginseng Res Date: 2017-01-25 Impact factor: 6.060