Xian-Ning Liu1, Xiu-Ping Zhu1, Jie Wu2, Zheng-Jie Wu3, Yong Yin1, Xiang-Hua Xiao1, Xin Su3, Bin Kong3, Shi-Yin Pan1, Hua Yang1, Yan Cheng2, Na An1, Sheng-Li Mi3. 1. Shaanxi Institute of Ophthalmology, Xi'an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi'an 710002, Shaanxi Province, China; Xi'an First Hospital, Xi'an 710002, Shaanxi Province, China. 2. Xi'an First Hospital, Xi'an 710002, Shaanxi Province, China. 3. Biomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong Province, China.
Abstract
AIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS: A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy (TEM) and hematoxylin and eosin (H&E) staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype. RESULTS: The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process. In vitro, the methyl thiazolyl tetrazolium results revealed that extracts of the acellular ostrich corneas (AOCs) had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes. The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea (APC) transplantation. The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer. CONCLUSION: We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft.
AIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS: A hypertonicsaline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy (TEM) and hematoxylin and eosin (H&E) staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype. RESULTS: The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process. In vitro, the methyl thiazolyl tetrazolium results revealed that extracts of the acellular ostrich corneas (AOCs) had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes. The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea (APC) transplantation. The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer. CONCLUSION: We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft.
Authors: Pedro L Sánchez; M Eugenia Fernández-Santos; Salvatore Costanza; Andreu M Climent; Isabel Moscoso; M Angeles Gonzalez-Nicolas; Ricardo Sanz-Ruiz; Hugo Rodríguez; Stefan M Kren; Gregorio Garrido; Jose L Escalante; Javier Bermejo; Jaime Elizaga; Javier Menarguez; Raquel Yotti; Candelas Pérez del Villar; M Angeles Espinosa; María S Guillem; James T Willerson; Antonio Bernad; Rafael Matesanz; Doris A Taylor; Francisco Fernández-Avilés Journal: Biomaterials Date: 2015-05-13 Impact factor: 12.479
Authors: Yi Shao; Yao Yu; Chong-Gang Pei; Qiong Zhou; Qiu-Ping Liu; Gang Tan; Jing-Ming Li; Gui-Ping Gao; Lu Yang Journal: Int J Ophthalmol Date: 2012-08-18 Impact factor: 1.779