Selected metabolic biochemical and enzyme activities associated with Besnoitia besnoiti infection in dairy cattle.

The main aim of the study was to explore, compare, and identify whether there is an association between Besnoitia besnoiti seropositivity in apparently healthy dairy cows with some biochemical parameters, enzyme activities, and beta-hydroxybutyrate (BHBA). A total of 98 dairy cows were included in the study, of which there was 50 seropositive and 48 seronegative cows. Analysis of serum antibodies against B. besnoiti antibodies was performed using an indirect enzyme-linked immunosorbent assay kit. Student's independent t test showed that there was a significant difference in BHBA, albumin, and lactate dehydrogenase (LDH) between the seropositive and seronegative groups. Univariable regression analysis showed no significant association between seropositivity status with any of the evaluated parameters except BHBA level, mastitis, and abomasum displacement. Multivariable logistic regression analysis showed that there was a strong association between seropositivity with BHBA level. The significant association between BHBA and B. besnoiti seropositivity represents preliminary finding that needs further exploration.