Literature DB >> 2715322

Synthetic exfoliative toxin A and B DNA probes for detection of toxigenic Staphylococcus aureus strains.

S Rifai1, V Barbancon, G Prevost, Y Piemont.   

Abstract

Two methods for the detection of exfoliative toxin (ET) from Staphylococcus aureus were compared: (i) a phenotypic assay, electrosyneresis, and (ii) a genotypic assay, staphylococcal DNA hybridization with oligodeoxynucleotide probes. The probes were chosen from the previously determined sequences of serotype A and B of ET, one probe for serotype A and another for serotype B. Strains exhibiting ET production in electrosyneresis always possessed the ET gene(s). Conversely, some strains not exhibiting ET production in electrosyneresis harbored the ET gene(s). The latter strains produced levels of ET. ET-negative phage group 2 strains of S. aureus as well as tested coagulase-negative staphylococci did not possess the ET gene(s). The sensitivity of the DNA hybridization technique was 10(6) bacteria or 100 ng of genomic DNA.

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Year:  1989        PMID： 2715322      PMCID： PMC267347          DOI： 10.1128/jcm.27.3.504-506.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

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Journal:  Clin Exp Dermatol       Date:  1978-03       Impact factor: 3.470

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Journal:  Infect Immun       Date:  1971-11       Impact factor: 3.441

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Authors:  K D Wuepper; D H Baker; R L Dimond
Journal:  J Invest Dermatol       Date:  1976-10       Impact factor: 8.551

5.  Enzyme-linked immunosorbent assays for Staphylococcus aureus exfoliative toxins A and B and some applications.

Authors:  Y Piémont; M Haubensack; H Monteil
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948

6.  [Prevalence of exfoliatin-producing strains in phage group II "Staphylococcus aureus" (author's transl)].

Authors:  J Fleurette; J Ritter
Journal:  Ann Microbiol (Paris)       Date:  1980 Sep-Oct

7.  [Exfoliatin in neonatal staphylococcal infections].

Authors:  D Willard; H Monteil; Y Piemont; R Assi; J Messer; J Lavillaureix; R Minck; R Gandar
Journal:  Nouv Presse Med       Date:  1982-12-18

8.  Nucleotide sequence of the epidermolytic toxin A gene of Staphylococcus aureus.

Authors:  P W O'Toole; T J Foster
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

9.  Qualitative and quantitative methods for detecting staphylococcal epidermolytic toxin.

Authors:  J P Arbuthnott; B Billcliffe
Journal:  J Med Microbiol       Date:  1976-05       Impact factor: 2.472

10.  [Demonstration by electrosyneresis of 2 exfoliation serotypes produced by Staphylococcus aureus].

Authors:  Y Piémont; H Monteil
Journal:  Ann Microbiol (Paris)       Date:  1983 Mar-Apr
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  12 in total

1.  Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction.

Authors:  W M Johnson; S D Tyler; E P Ewan; F E Ashton; D R Pollard; K R Rozee
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

2.  Enterotoxigenic potential of Staphylococcus intermedius.

Authors:  K Becker; B Keller; C von Eiff; M Brück; G Lubritz; J Etienne; G Peters
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

3.  Staphylococcus aureus isolated in cases of impetigo produces both epidermolysin A or B and LukE-LukD in 78% of 131 retrospective and prospective cases.

Authors:  A Gravet; P Couppié; O Meunier; E Clyti; B Moreau; R Pradinaud; H Monteil; G Prévost
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

4.  Sequencing of leucocidin R from Staphylococcus aureus P83 suggests that staphylococcal leucocidins and gamma-hemolysin are members of a single, two-component family of toxins.

Authors:  G Supersac; G Prevost; Y Piemont
Journal:  Infect Immun       Date:  1993-02       Impact factor: 3.441

5.  Frequency of isolation of Staphylococcus intermedius from humans.

Authors:  I Mahoudeau; X Delabranche; G Prevost; H Monteil; Y Piemont
Journal:  J Clin Microbiol       Date:  1997-08       Impact factor: 5.948

6.  Exfoliative toxin detection using reversed passive latex agglutination: clinical and epidemiologic applications.

Authors:  A Kawabata; S Ichiyama; Y Iinuma; Y Hasegawa; M Ohta; K Shimokata
Journal:  J Clin Microbiol       Date:  1997-08       Impact factor: 5.948

Review 7.  Clinical, microbial, and biochemical aspects of the exfoliative toxins causing staphylococcal scalded-skin syndrome.

Authors:  S Ladhani; C L Joannou; D P Lochrie; R W Evans; S M Poston
Journal:  Clin Microbiol Rev       Date:  1999-04       Impact factor: 26.132

8.  Sensitive and specific detection of staphylococcal epidermolysins A and B in broth cultures by flow cytometry-assisted multiplex immunoassay.

Authors:  Olivier Joubert; Daniel Keller; Anne Pinck; Henri Monteil; Gilles Prévost
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

9.  Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene.

Authors:  K Becker; R Roth; G Peters
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

10.  Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus.

Authors:  K M Cregg; I Wilding; M T Black
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490
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