| Literature DB >> 27151371 |
Harry M Murray1, Stephen J Hill2, Keng P Ang3.
Abstract
The description and application of a modified Scanning Electron Microscope preparation technique using hexamethyldisilazane for small parasitic copepods was demonstrated though a high resolution depiction of individuals of Ergasilus labracis sampled from three spined stickleback (Gasterosteus aculeatus) in Bay D'Espoir, Newfoundland during summer 2015 and from archival samples retrieved from Atlantic salmon par (Salmo salar) stored at the Atlantic reference centre, St. Andrews, New Brunswick. The specimens were very well preserved showing high quality detail of important features and verifying those previously described using light microscopy by Hogans. Additionally the technique allowed excellent in situ demonstrations of mouth parts, swimming legs, and unusual and previously undescribed features of the second antenna including prominent striations and pore-like structures found to define the claw. It is thought that this technique will become a quick and efficient tool for describing important taxonomic features of small parasitic copepods like E. labracis or other similar small aquatic organisms. Microsc. Res. Tech. 79:657-663, 2016.Entities:
Keywords: HMDS; antennae; mandible; parasitic copepod; surface striation; swimming legs
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Year: 2016 PMID: 27151371 DOI: 10.1002/jemt.22680
Source DB: PubMed Journal: Microsc Res Tech ISSN: 1059-910X Impact factor: 2.769