| Literature DB >> 27150590 |
Fabrizia Veronesi1, Silvia Ravagnan2, Matteo Cerquetella3, Erika Carli2, Emanuela Olivieri4, Azzurra Santoro4, Stefano Pesaro5, Sara Berardi3, Giacomo Rossi3, Bernardino Ragni6, Paola Beraldo7, Gioia Capelli2.
Abstract
Cytauxzoonosis is an emerging, tick-transmitted, protozoan disease affecting domestic and wild felids and caused by Cytauxzoon felis, Cytauxzoon manul and Cytauxzoon spp. This study aimed to determine the presence of infection with Cytauxzoon spp. in Felis silvestris silvestris in Italy, in order to enhance the comprehension of its pattern distribution among domestic cat populations. In addition, wildcats were tested for other endemic vector-borne pathogens in Italy. The carcasses of 21 F. s. silvestris were collected from central and northern regions of Italy. All the animals were submitted to necropsy and samples of the spleens were collected. Cytauxzoon infection was surveyed by a conventional PCR amplifying a portion of the SSU-rDNA of species of Piroplasmida. The samples were also screened for Anaplasma spp., Ehrlichia spp., Rickettsia spp., Babesia spp., Theileria spp., and Leishmania spp. using SYBR Green Real-Time PCR (rPCR) assays. Four animals (19%) were positive for Piroplasmida-PCR assay and three sequenced amplicons were obtained (14.3%), clustering with the Italian, Spanish, French and Romanian Cytauxzoon spp. isolates and with C. manul found in Mongolia. The samples were negative for the other pathogens screened. The present results showed that Cytauxzoon spp. may infect both F. s. silvestris and F. s. catus.Entities:
Keywords: Cytauxzoon spp; Felis silvestris silvestris; Italy; Vector-borne pathogens
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Year: 2016 PMID： 27150590 DOI： 10.1016/j.ttbdis.2016.04.003
Source DB: PubMed Journal: Ticks Tick Borne Dis ISSN： 1877-959X Impact factor: 3.744