Literature DB >> 2715053

Optimization of the histochemical demonstration of DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B.

G Nöhammer1.   

Abstract

Previous methods for the histochemical demonstration of DNA were optimized. p-Toluene sulfonic acid as catalyst for hydrazone formation between the aldehydes generated after Feulgen hydrolysis and 3-hydroxy-2-naphthoic acid hydrazide (NAH) was used instead of acetic acid. Modifications of the conditions of the coupling reaction with Fast Blue B reduced the background staining. The optimized histochemical staining method for DNA (NAH-FB-DNA staining) can be performed easily and reproducibly. Without prior Feulgen hydrolysis the optimized method can also be used for the histochemical demonstration of reactive carbonyls undissolved under the given histochemical conditions.

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Year:  1989        PMID： 2715053     DOI： 10.1007/BF00494358

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  13 in total

1.  [Acid hydrolysis of ribonucleic acid and desoxyribonucleic acid].

Authors:  W POLLMANN; G SCHRAMM
Journal:  Z Naturforsch B       Date:  1961-10       Impact factor: 1.047

2.  A Review of Modern Methods in Histochemistry.

Authors:  A G Pearse
Journal:  J Clin Pathol       Date:  1951-02       Impact factor: 3.411

3.  A new reagent for the histochemical demonstration of active carbonyl groups; a new method for staining ketonic steroids.

Authors:  R ASHBEL; A M SELIGMAN
Journal:  Endocrinology       Date:  1949-06       Impact factor: 4.736

4.  Feulgen nucleal reaction. II. Approaches to the understanding of mechanism of in vitro reaction.

Authors:  T Hiraoka
Journal:  Histochemie       Date:  1973-06-29

5.  Application of the method of kinetic analysis of staining and destaining processes to the complex formed between hydrolyzed deoxyribonucleoprotein and Schiff's reagent in model films.

Authors:  W A Duijndam; J Hermans; P van Duijn
Journal:  J Histochem Cytochem       Date:  1973-08       Impact factor: 2.479

6.  [Quantitative histochemical detection of sulfhdryl groups using the DDD staining. I. Studies of the dyes].

Authors:  H Esterbauer
Journal:  Acta Histochem       Date:  1972       Impact factor: 2.479

7.  The cytospectrophotometrical determination of proteins by the tetrazonium coupling reaction. II. Calibration of the staining method.

Authors:  G Nöhammer; G Desoye
Journal:  Acta Histochem       Date:  1981       Impact factor: 2.479

8.  [The cytospectrophotometrical determination of proteins by the tetrazonium coupling reaction. I. Optimization of the staining method (author's transl)].

Authors:  G Nöhammer
Journal:  Acta Histochem       Date:  1978       Impact factor: 2.479

9.  Quantitative microspectrophotometrical determination of protein thiols and disulfides with 2,2'-dihydroxy-6,6'-dinaphthyldisulfide (DDD). The variety of DDD-staining methods demonstrated on Ehrlich ascites tumor cells.

Authors:  G Nöhammer
Journal:  Histochemistry       Date:  1982

10.  A chromatographic study of hydrolysis in the Feulgen nucleal reaction.

Authors:  P S WOODS
Journal:  J Biophys Biochem Cytol       Date:  1957-01-25
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